Acyl-CoA thioesterase 8 is a specific protein related to nodal metastasis and prognosis of lung adenocarcinoma

Woon Yong Jung, Young Hye Kim, Young Joon Ryu, Baek Hui Kim, Bong Kyung Shin, Aeree Kim, Han Kyeom Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Metastasis is a major cause of cancer recurrence or death. This study attempted to quantitatively identify different proteins in metastatic lung adenocarcinoma. The N/. T quotient [number of metastatic lymph nodes (n)/tumor diameter (cm)] was used to select samples with an extreme metastatic phenotype. Among the six fresh frozen lung adenocarcinoma specimens, the three showing the highest N/. T quotient represented the metastatic group, and others with the greatest tumor diameters without metastasis represented the non-metastatic group. After 2-dimensional electrophoresis, the significantly different protein spots were selected by image analysis and analyzed with MALDI-TOF mass spectrometry. Acyl-CoA thioesterase 8 isoform c (ACOT8) was one of most overexpressed proteins in the metastatic group, and it was validated by Western blot and immunohistochemical staining on 108 paraffin-embedded tumor samples. High ACOT8 expression was correlated with lymph node metastasis (p = 0.002), recurrence (p = 0.034), predominant histologic subtypes (p = 0.007), and higher stage (p = 0.005). In multivariate analysis, high ACOT8 expression was significantly associated with increased risks of lymph node metastasis (p = 0.009) and cancer-related death (p = 0.030), independent of clinical factors. ACOT8 may be a candidate prognostic biomarker and therapeutic target of lung adenocarcinoma.

Original languageEnglish
Pages (from-to)276-283
Number of pages8
JournalPathology Research and Practice
Volume209
Issue number5
DOIs
Publication statusPublished - 2013 May

Keywords

  • Adenocarcinoma
  • Lung
  • Neoplasm metastasis
  • Prognosis
  • Proteomics

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology

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