Acyl-CoA thioesterase 8 is a specific protein related to nodal metastasis and prognosis of lung adenocarcinoma

Woon Yong Jung, Young Hye Kim, Young Joon Ryu, Baek-Hui Kim, Bong Kyung Shin, Aeree Kim, Han Kyeom Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Metastasis is a major cause of cancer recurrence or death. This study attempted to quantitatively identify different proteins in metastatic lung adenocarcinoma. The N/. T quotient [number of metastatic lymph nodes (n)/tumor diameter (cm)] was used to select samples with an extreme metastatic phenotype. Among the six fresh frozen lung adenocarcinoma specimens, the three showing the highest N/. T quotient represented the metastatic group, and others with the greatest tumor diameters without metastasis represented the non-metastatic group. After 2-dimensional electrophoresis, the significantly different protein spots were selected by image analysis and analyzed with MALDI-TOF mass spectrometry. Acyl-CoA thioesterase 8 isoform c (ACOT8) was one of most overexpressed proteins in the metastatic group, and it was validated by Western blot and immunohistochemical staining on 108 paraffin-embedded tumor samples. High ACOT8 expression was correlated with lymph node metastasis (p = 0.002), recurrence (p = 0.034), predominant histologic subtypes (p = 0.007), and higher stage (p = 0.005). In multivariate analysis, high ACOT8 expression was significantly associated with increased risks of lymph node metastasis (p = 0.009) and cancer-related death (p = 0.030), independent of clinical factors. ACOT8 may be a candidate prognostic biomarker and therapeutic target of lung adenocarcinoma.

Original languageEnglish
Pages (from-to)276-283
Number of pages8
JournalPathology Research and Practice
Volume209
Issue number5
DOIs
Publication statusPublished - 2013 May 1

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Acyl Coenzyme A
Neoplasm Metastasis
Protein Isoforms
Lymph Nodes
Neoplasms
Proteins
Recurrence
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Paraffin
Electrophoresis
Mass Spectrometry
Multivariate Analysis
Biomarkers
Western Blotting
Adenocarcinoma of lung
Staining and Labeling
Phenotype

Keywords

  • Adenocarcinoma
  • Lung
  • Neoplasm metastasis
  • Prognosis
  • Proteomics

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology

Cite this

Acyl-CoA thioesterase 8 is a specific protein related to nodal metastasis and prognosis of lung adenocarcinoma. / Jung, Woon Yong; Kim, Young Hye; Ryu, Young Joon; Kim, Baek-Hui; Shin, Bong Kyung; Kim, Aeree; Kim, Han Kyeom.

In: Pathology Research and Practice, Vol. 209, No. 5, 01.05.2013, p. 276-283.

Research output: Contribution to journalArticle

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