ADAM10 inhibits the interaction between IL-17 and HMGB1 in Buerger’s disease

J. K. Byun, K. H. Kim, S. W. Rha, Y. Park, J. A. Cha, S. Y. Choi, B. G. Choi, Cheol Ung Choi

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVE: Buerger’s disease is a rare disease that causes critical limb ischemia; however, the underlying pathophysiologi-cal mechanism remains unclear. Therefore, we investigated the interaction between interleukin (IL)-17 and high-mobility group protein B 1 (HMGB1) and determined whether A disintegrin and metalloproteinase 10 (ADAM10) inhibit this interaction. PATIENTS AND METHODS: The study population included 15 patients with Buerger’s disease and 10 healthy donors without a history of giving peripheral blood samples. Cytokine levels were measured using a luminex multiplex assay in plasma. Flow cytometry was used to analyze the subtypes of helper T (Th) cells among peripheral blood mononuclear cells (PBMCs). The effect of ADAM10 on PBMCs was analyzed in vitro. RESULTS: The levels of inflammatory cytokines and production of pathogenic Th cells were found to be higher in Korean patients with Buerger’s disease. IL-17 treatment induced HMGB1 associated molecules. HMGB1 also induced IL-17 and Th17 associated transcription factors in Buerger’s patients. We observed that ADAM10 regulates the interaction between IL-17 and HMGB1 via advanced glycation end products (RAGE)/nuclear factor-kappa B (NF-kB) pathway in patients with Buerger’s disease. CONCLUSIONS: This study suggests that IL-17 and HMGB1 cytokines contribute to the pathogenesis of Buerger’s disease. These results indicate that ADAM10 alleviates inflammation in Buerger’s disease via the HMGB1 and RAGE/NF-κB signaling pathway and provides insights into the molecular basis of and a potential therapeutic strategy for Buerger’s disease.

Original languageEnglish
Pages (from-to)4051-4063
Number of pages13
JournalEuropean Review for Medical and Pharmacological Sciences
Volume25
Issue number11
DOIs
Publication statusPublished - 2021

Keywords

  • ADAM10
  • Buerger’s disease
  • HMGB1
  • IL-17

ASJC Scopus subject areas

  • Pharmacology (medical)

Fingerprint

Dive into the research topics of 'ADAM10 inhibits the interaction between IL-17 and HMGB1 in Buerger’s disease'. Together they form a unique fingerprint.

Cite this