Adaptive responses of Escherichia coli for oxidative and protein damage using bioluminescence reporters

Jiho Min, Man Bock Gu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The recombinant bioluminescent Escherichia coli strains, DPD2511 and TV1061 containing the katG and grpE promoters, respectively, from Vibrio fischeri fused to luxCDABE, were used to detect the adaptive and repair responses to oxidative damage caused by hydrogen peroxide (H2O2), and protein damage due to phenol. The response ratio, represented as the bioluminescence induced in subsequent inductions of DPD2511 and TV1061 with the mother cells previously induced by each chemical, i.e., H2O2 and phenol during the previous induction stage, decreased suddenly compared with the ratio of the control culture of each strain, meaning there is a possible adaptive response to stress caused by chemicals. Protein damage due to phenol was completely repaired by the second culturing after the initial induction, as was oxidative damage caused by H2O2, which was also rapidly repaired, as detected by the recovery of bioluminescence level. This result suggests that E. coli promptly adapt and repair oxidative and protein damage by H2O2 and phenol completely.

Original languageEnglish
Pages (from-to)466-469
Number of pages4
JournalJournal of microbiology and biotechnology
Volume14
Issue number3
Publication statusPublished - 2004 Jun 1
Externally publishedYes

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Keywords

  • Oxidative damage
  • Protein damage
  • Stress adaptation
  • grpE promoter
  • katG promoter

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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