Adenovirus-mediated antisense expression of telomerase template RNA induces apoptosis in lung cancer cells

A. Song, Joon Seok, Sang Bae Kim, Young Ho Lee, Kyu Wan Lee, Hak Hyun Jung, Mee Hye Kim, Kyung Tai Kim, Robert Brown, Young Tae Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Telomerase is a ribonucleoprotein complex, whose function is to add telomeric repeats (TTAGGG)n to chromosomal ends and is also known to play an important role in cellular immortalization. Telomerase is highly active in most tumor cells, yet not in normal cells. Therefore, it may have possible applications in cancer gene therapy. Telomerase consists of two essential components; a telomerase RNA template (hTR) and a catalytic subunit (hTERT). The current study attempted to inhibit the "open" part of the human telomerase RNA (hTR) with an antisense sequence-expressing adenovirus. It was found that the antisense telomerase adenovirus suppressed the telomerase activity, tumor cell growth, and survival in vitro. Furthermore, FACS analysis and TUNEL assay suggested that the reduced viability was mediated through the induction of apoptosis, indicating that this approach might be a useful method for suppressing cancer growth in targeted cancer gene therapy.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume12
Issue number1
Publication statusPublished - 2002 Apr 15

Fingerprint

Telomerase
Adenoviridae
Lung Neoplasms
Apoptosis
Neoplasm Genes
Genetic Therapy
Neoplasms
Ribonucleoproteins
In Situ Nick-End Labeling
Growth
Catalytic Domain
Cell Survival
telomerase RNA

Keywords

  • Adenovirus
  • Antisense sequence
  • hTR
  • Lung cancer cell
  • Targeted cancer gene therapy
  • Telomerase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Adenovirus-mediated antisense expression of telomerase template RNA induces apoptosis in lung cancer cells. / Song, A.; Seok, Joon; Kim, Sang Bae; Lee, Young Ho; Lee, Kyu Wan; Jung, Hak Hyun; Kim, Mee Hye; Kim, Kyung Tai; Brown, Robert; Kim, Young Tae.

In: Journal of Microbiology and Biotechnology, Vol. 12, No. 1, 15.04.2002, p. 89-95.

Research output: Contribution to journalArticle

Song, A, Seok, J, Kim, SB, Lee, YH, Lee, KW, Jung, HH, Kim, MH, Kim, KT, Brown, R & Kim, YT 2002, 'Adenovirus-mediated antisense expression of telomerase template RNA induces apoptosis in lung cancer cells', Journal of Microbiology and Biotechnology, vol. 12, no. 1, pp. 89-95.
Song, A. ; Seok, Joon ; Kim, Sang Bae ; Lee, Young Ho ; Lee, Kyu Wan ; Jung, Hak Hyun ; Kim, Mee Hye ; Kim, Kyung Tai ; Brown, Robert ; Kim, Young Tae. / Adenovirus-mediated antisense expression of telomerase template RNA induces apoptosis in lung cancer cells. In: Journal of Microbiology and Biotechnology. 2002 ; Vol. 12, No. 1. pp. 89-95.
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AU - Jung, Hak Hyun

AU - Kim, Mee Hye

AU - Kim, Kyung Tai

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