Adipogenic and lipolytic effects of ascorbic acid in ovariectomized rats

Byoungjae Kim, Kyung Min Choi, Hong Soon Yim, Hyun-Tae Park, Joung Han Yim, Min-Goo Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: Ascorbic acid has been reported to have an adipogenic effect on 3T3-L1 preadipocytes, while evidence also suggests that ascorbic acid reduces body weight in humans. In this study, we tested the effects of ascorbic acid on adipogenesis and the balance of lipid accumulation in ovariectomized rats, in addition to long-term culture of differentiated 3T3-L1 adipocytes. Materials and Methods: Murine 3T3-L1 fibroblasts and ovariectomized rats were treated with ascorbic acid at various time points. In vitro adipogenesis was analyzed by Oil Red O staining, and in vivo body fat was measured by a body composition analyzer using nuclear magnetic resonance. Results: When ascorbic acid was applied during an early time point in 3T3-L1 preadipocyte differentiation and after bilateral ovariectomy (OVX) in rats, adipogenesis and fat mass gain significantly increased, respectively. However, lipid accumulation in well-differentiated 3T3-L1 adipocytes showed a significant reduction when ascorbic acid was applied after differentiation (10 days after induction). Also, oral ascorbic acid administration 4 weeks after OVX in rats significantly reduced both body weight and subcutaneous fat layer. In comparison to the results of ascorbic acid, which is a well-known cofactor for an enzyme of collagen synthesis, and the antioxidant ramalin, a potent antioxidant but not a cofactor, showed only a lipolytic effect in well-differentiated 3T3-L1 adipocytes, not an adipogenic effect. Conclusion: Taking these results into account, we concluded that ascorbic acid has both an adipogenic effect as a cofactor of an enzymatic process and a lipolytic effect as an antioxidant.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalYonsei Medical Journal
Volume59
Issue number1
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

Ascorbic Acid
Adipogenesis
Adipocytes
Antioxidants
Body Weight
Lipids
Subcutaneous Fat
Coenzymes
Ovariectomy
Body Composition
Adipose Tissue
Magnetic Resonance Spectroscopy
Collagen
Fibroblasts
Fats
Staining and Labeling

Keywords

  • 3T3-L1 cell
  • Adipogenesis
  • Ascorbic acid
  • Lipolysis
  • Obesity
  • Ovariectomy

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Adipogenic and lipolytic effects of ascorbic acid in ovariectomized rats. / Kim, Byoungjae; Choi, Kyung Min; Yim, Hong Soon; Park, Hyun-Tae; Yim, Joung Han; Lee, Min-Goo.

In: Yonsei Medical Journal, Vol. 59, No. 1, 01.01.2018, p. 85-91.

Research output: Contribution to journalArticle

Kim, Byoungjae ; Choi, Kyung Min ; Yim, Hong Soon ; Park, Hyun-Tae ; Yim, Joung Han ; Lee, Min-Goo. / Adipogenic and lipolytic effects of ascorbic acid in ovariectomized rats. In: Yonsei Medical Journal. 2018 ; Vol. 59, No. 1. pp. 85-91.
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