Adipose gene expression profiles related to metabolic syndrome using microarray analyses in two different models

Hye-Jin Yoo, Hwan Jin Hwang, Tae Woo Jung, Ja Young Ryu, Ho Cheol Hong, Hae Yoon Choi, Sei-Hyun Baik, Kyung Mook Choi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Peroxisome proliferator-activated receptor-γ (PPAR-γ) agonist has a wide-ranging influence on multiple components of metabolic syndrome. The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is a useful animal model of metabolic syndrome. To determine genes related to metabolic syndrome, we examined overlapping genes that are simultaneously decreased by PPAR-γ agonists and increased in OLETF rats using microarrays in two different models. Methods: In the first microarray analysis, PPAR-γ agonist-treated db/db mice were compared to standard diet-fed db/db mice. In the second microarray analysis, OLETF rats were compared to Long-Evans Tokushima Otsuka (LETO) rats (control of OLETF rats). Results: Among the overlapping genes, in the present study, we validated that lipocalin-2 expression was significantly decreased in the visceral adipose tissue of PPAR-γ agonist-treated db/db mice compared to standard diet-fed db/db mice and increased in OLETF rats compared to LETO rats using real time reverse transcription polymerase chain reaction. Furthermore, we showed for the first time that lipocalin-2 expression was significantly increased in the visceral adipose tissues of obese humans compared with nonobese humans. In addition, the expression level of lipocalin-2 in human visceral adipose tissue had a significant positive correlation with body mass index, serum interleukin-6, adipocyte fatty acid binding protein levels, and white blood cell count. Conclusion: Lipocalin-2 was confirmed to be a significant adipokine affected by PPAR-γ agonist and obesity in the present study. Also, for the first time in human visceral adipose tissue, it was determined that the expression of lipocalin-2 from obese humans was significantly increased and correlated with circulating inflammatory markers.

Original languageEnglish
Pages (from-to)356-365
Number of pages10
JournalDiabetes and Metabolism Journal
Volume38
Issue number5
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Inbred OLETF Rats
Microarray Analysis
Transcriptome
Peroxisome Proliferator-Activated Receptors
Intra-Abdominal Fat
Overlapping Genes
Diet
Fatty Acid-Binding Proteins
Adipokines
Leukocyte Count
Adipocytes
Reverse Transcription
Interleukin-6
Body Mass Index
Animal Models
Obesity
Lipocalin-2
Polymerase Chain Reaction

Keywords

  • Lipocalin-2
  • Microarray
  • PPAR gamma

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

Cite this

Adipose gene expression profiles related to metabolic syndrome using microarray analyses in two different models. / Yoo, Hye-Jin; Hwang, Hwan Jin; Jung, Tae Woo; Ryu, Ja Young; Hong, Ho Cheol; Choi, Hae Yoon; Baik, Sei-Hyun; Choi, Kyung Mook.

In: Diabetes and Metabolism Journal, Vol. 38, No. 5, 01.01.2014, p. 356-365.

Research output: Contribution to journalArticle

Yoo, Hye-Jin ; Hwang, Hwan Jin ; Jung, Tae Woo ; Ryu, Ja Young ; Hong, Ho Cheol ; Choi, Hae Yoon ; Baik, Sei-Hyun ; Choi, Kyung Mook. / Adipose gene expression profiles related to metabolic syndrome using microarray analyses in two different models. In: Diabetes and Metabolism Journal. 2014 ; Vol. 38, No. 5. pp. 356-365.
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