Adipose tissue formation utilizing fat flap distraction technique

Myung Chul Lee, Won Jai Lee, Byung-Il Lee, Kee Yang Chung, Jae Woo Kim, Eun Hye Kang, Yong Oock Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Co-regulation between adipocytes and supporting vasculature is considered an important process in adipose tissue generation. The objective of this study was to evaluate the mechanical and biological effects of a distraction technique on adipose tissue formation and maintenance. Based on the hypothesis that fat flaps gradually receding from each other can develop an adipose tissue construct, perforated polycarbonate syringe-shaped chambers were implanted in a rabbit model. Latency (1 week) and distraction (3 weeks) periods were followed by a consolidation period in the experimental groups (4, 8, and 12 weeks). In the distraction group, the volume of fat pad gradually increased up to 16 weeks. A transition zone was observed at 8 weeks, indicating the initiation of tissue generation. Histomorphologic analysis showed adipose and collagen connective tissue at 8 weeks. At 16 weeks, the relative composition was altered significantly. Adipose components occupied most of the tissue, and connective tissue was reduced. Blood vessels with endothelial lining were noted adjacent to adipocyte clusters, as well as in inter-adipocyte areas. The vessels had increased in number and were evenly distributed by 16 weeks. Our distraction technique produced more balanced adipose tissue generation than a non-distraction method, with co-development of adipose and vascular tissues.

Original languageEnglish
Article number5174
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

Fingerprint

Adipose Tissue
Fats
Adipocytes
polycarbonate
Connective Tissue
Blood Vessels
Syringes
Collagen
Maintenance
Rabbits

ASJC Scopus subject areas

  • General

Cite this

Lee, M. C., Lee, W. J., Lee, B-I., Chung, K. Y., Kim, J. W., Kang, E. H., & Kim, Y. O. (2017). Adipose tissue formation utilizing fat flap distraction technique. Scientific Reports, 7(1), [5174]. https://doi.org/10.1038/s41598-017-05547-y

Adipose tissue formation utilizing fat flap distraction technique. / Lee, Myung Chul; Lee, Won Jai; Lee, Byung-Il; Chung, Kee Yang; Kim, Jae Woo; Kang, Eun Hye; Kim, Yong Oock.

In: Scientific Reports, Vol. 7, No. 1, 5174, 01.12.2017.

Research output: Contribution to journalArticle

Lee, MC, Lee, WJ, Lee, B-I, Chung, KY, Kim, JW, Kang, EH & Kim, YO 2017, 'Adipose tissue formation utilizing fat flap distraction technique', Scientific Reports, vol. 7, no. 1, 5174. https://doi.org/10.1038/s41598-017-05547-y
Lee, Myung Chul ; Lee, Won Jai ; Lee, Byung-Il ; Chung, Kee Yang ; Kim, Jae Woo ; Kang, Eun Hye ; Kim, Yong Oock. / Adipose tissue formation utilizing fat flap distraction technique. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
@article{49bc88bc2c984df49521339df76056c6,
title = "Adipose tissue formation utilizing fat flap distraction technique",
abstract = "Co-regulation between adipocytes and supporting vasculature is considered an important process in adipose tissue generation. The objective of this study was to evaluate the mechanical and biological effects of a distraction technique on adipose tissue formation and maintenance. Based on the hypothesis that fat flaps gradually receding from each other can develop an adipose tissue construct, perforated polycarbonate syringe-shaped chambers were implanted in a rabbit model. Latency (1 week) and distraction (3 weeks) periods were followed by a consolidation period in the experimental groups (4, 8, and 12 weeks). In the distraction group, the volume of fat pad gradually increased up to 16 weeks. A transition zone was observed at 8 weeks, indicating the initiation of tissue generation. Histomorphologic analysis showed adipose and collagen connective tissue at 8 weeks. At 16 weeks, the relative composition was altered significantly. Adipose components occupied most of the tissue, and connective tissue was reduced. Blood vessels with endothelial lining were noted adjacent to adipocyte clusters, as well as in inter-adipocyte areas. The vessels had increased in number and were evenly distributed by 16 weeks. Our distraction technique produced more balanced adipose tissue generation than a non-distraction method, with co-development of adipose and vascular tissues.",
author = "Lee, {Myung Chul} and Lee, {Won Jai} and Byung-Il Lee and Chung, {Kee Yang} and Kim, {Jae Woo} and Kang, {Eun Hye} and Kim, {Yong Oock}",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41598-017-05547-y",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Adipose tissue formation utilizing fat flap distraction technique

AU - Lee, Myung Chul

AU - Lee, Won Jai

AU - Lee, Byung-Il

AU - Chung, Kee Yang

AU - Kim, Jae Woo

AU - Kang, Eun Hye

AU - Kim, Yong Oock

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Co-regulation between adipocytes and supporting vasculature is considered an important process in adipose tissue generation. The objective of this study was to evaluate the mechanical and biological effects of a distraction technique on adipose tissue formation and maintenance. Based on the hypothesis that fat flaps gradually receding from each other can develop an adipose tissue construct, perforated polycarbonate syringe-shaped chambers were implanted in a rabbit model. Latency (1 week) and distraction (3 weeks) periods were followed by a consolidation period in the experimental groups (4, 8, and 12 weeks). In the distraction group, the volume of fat pad gradually increased up to 16 weeks. A transition zone was observed at 8 weeks, indicating the initiation of tissue generation. Histomorphologic analysis showed adipose and collagen connective tissue at 8 weeks. At 16 weeks, the relative composition was altered significantly. Adipose components occupied most of the tissue, and connective tissue was reduced. Blood vessels with endothelial lining were noted adjacent to adipocyte clusters, as well as in inter-adipocyte areas. The vessels had increased in number and were evenly distributed by 16 weeks. Our distraction technique produced more balanced adipose tissue generation than a non-distraction method, with co-development of adipose and vascular tissues.

AB - Co-regulation between adipocytes and supporting vasculature is considered an important process in adipose tissue generation. The objective of this study was to evaluate the mechanical and biological effects of a distraction technique on adipose tissue formation and maintenance. Based on the hypothesis that fat flaps gradually receding from each other can develop an adipose tissue construct, perforated polycarbonate syringe-shaped chambers were implanted in a rabbit model. Latency (1 week) and distraction (3 weeks) periods were followed by a consolidation period in the experimental groups (4, 8, and 12 weeks). In the distraction group, the volume of fat pad gradually increased up to 16 weeks. A transition zone was observed at 8 weeks, indicating the initiation of tissue generation. Histomorphologic analysis showed adipose and collagen connective tissue at 8 weeks. At 16 weeks, the relative composition was altered significantly. Adipose components occupied most of the tissue, and connective tissue was reduced. Blood vessels with endothelial lining were noted adjacent to adipocyte clusters, as well as in inter-adipocyte areas. The vessels had increased in number and were evenly distributed by 16 weeks. Our distraction technique produced more balanced adipose tissue generation than a non-distraction method, with co-development of adipose and vascular tissues.

UR - http://www.scopus.com/inward/record.url?scp=85023617149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85023617149&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-05547-y

DO - 10.1038/s41598-017-05547-y

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 5174

ER -