Adsorption of microbial esterases on Bacillus subtilis-templated cobalt oxide nanoparticles

Eunjin Jang, Bum Han Ryu, Hyun Woo Shim, Hansol Ju, Dong-Wan Kim, T. Doohun Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Due to low diffusion rates and large surface areas, nanomaterials have received great interest as supporting materials for enzyme immobilization. Here, the preparation of a cobalt oxide nanoparticle using Bacillus subtilis as a biological template and use of the nanostructure for microbial esterase immobilization is described. Morphological features and size distributions were investigated using electron microscopy (EM) and dynamic light scattering (DLS). Catalytic properties of enzyme-coated nanostructures were investigated using 4-methylumbelliferyl acetate and p-nitrophenyl (PNP) acetate as model substrates. Enzyme-coated nanostructures were observed to retain ~85% of the initial activity after 15 successive reaction cycles, and enzyme immobilization processes could be repeated four times without a loss of immobilization potential. The present work demonstrates that B. subtilis-templated cobalt oxide nanoparticles have the potential to be used as biocompatible immobilization materials, and are promising candidates for the preparation of effective nanobiocatalysts.

Original languageEnglish
Pages (from-to)188-192
Number of pages5
JournalInternational Journal of Biological Macromolecules
Volume65
DOIs
Publication statusPublished - 2014 Apr 1
Externally publishedYes

Fingerprint

Bacilli
Esterases
Bacillus subtilis
Immobilization
Nanoparticles
Nanostructures
Enzyme immobilization
Adsorption
Enzymes
Dynamic light scattering
Nanostructured materials
Electron microscopy
Biocompatible Materials
Substrates
Electron Microscopy
cobalt oxide

Keywords

  • Bacillus subtilis
  • Cobalt-oxide nanoparticles
  • Hydrolase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Cite this

Adsorption of microbial esterases on Bacillus subtilis-templated cobalt oxide nanoparticles. / Jang, Eunjin; Ryu, Bum Han; Shim, Hyun Woo; Ju, Hansol; Kim, Dong-Wan; Kim, T. Doohun.

In: International Journal of Biological Macromolecules, Vol. 65, 01.04.2014, p. 188-192.

Research output: Contribution to journalArticle

Jang, Eunjin ; Ryu, Bum Han ; Shim, Hyun Woo ; Ju, Hansol ; Kim, Dong-Wan ; Kim, T. Doohun. / Adsorption of microbial esterases on Bacillus subtilis-templated cobalt oxide nanoparticles. In: International Journal of Biological Macromolecules. 2014 ; Vol. 65. pp. 188-192.
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