AEG-1 regulates TWIK-1 expression as an RNA-binding protein in astrocytes

Hyun Gug Jung, Ajung Kim, Seung Chan Kim, Jae Yong Park, Eun Mi Hwang

Research output: Contribution to journalArticlepeer-review

Abstract

AEG-1, also called MTDH, has oncogenic potential in numerous cancers and is considered a multifunctional modulator because of its involvement in developmental processes and inflammatory and degenerative brain diseases. However, the role of AEG-1 in astrocytes remains unknown. This study aimed to investigate proteins directly regulated by AEG-1 by analyzing their RNA expression patterns in astrocytes transfected with scramble shRNA and AEG-1 shRNA. AEG-1 knockdown down-regulated TWIK-1 mRNA. Real-time quantitative PCR (qPCR) and immunocytochemistry assays confirmed that AEG-1 modulates TWIK-1 mRNA and protein expression. Electrophysiological experiments further revealed that AEG-1 further regulates TWIK-1-mediated potassium currents in normal astrocytes. An RNA immunoprecipitation assay to determine how AEG-1 regulates the expression of TWIK-1 revealed that AEG-1 binds directly to TWIK-1 mRNA. Furthermore, TWIK-1 mRNA stability was significantly increased upon overexpression of AEG-1 in cultured astrocytes (p < 0.01). Our findings show that AEG-1 serves as an RNA-binding protein to regulate TWIK-1 expression in normal astrocytes.

Original languageEnglish
Article number85
Pages (from-to)1-11
Number of pages11
JournalBrain Sciences
Volume11
Issue number1
DOIs
Publication statusPublished - 2021 Jan

Keywords

  • AEG-1
  • Astrocyte
  • RNA-binding protein
  • TWIK-1

ASJC Scopus subject areas

  • Neuroscience(all)

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