Age- and vitamin E-induced changes in gene expression profiles of T cells

Sung Nim Hau, Oskar Adolfsson, Cheol-Koo Lee, Tomas A. Prolla, Jose Ordovas, Simin Nikbin Meydani

Research output: Contribution to journalArticle

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Abstract

T cells are vulnerable to age-associated changes. Vitamin E has been shown to improve T cell functions in the old. We studied gene expression profiles of T cells to better understand the underlying mechanisms of age and vitamin E-induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (supplemented) ppm of vitamin E for 4 wk. Gene expression profiles of T cells were assessed using microarray analysis with/without anti-CB3/anti-CB28 stimulation. Genes associated with cytokines/chemokines, transcriptional regulation, signal transduction, cell cycle, and apoptosis were significantly up-regulated upon stimulation. Higher SOCS3 and lower growth factor independent 1 (Gfi-1) expression in old T cells may contribute to age-associated decline in proliferation. Higher Gadd45 and lower Bcl2 expression may contribute to increased apoptosis in old T cells. Vitamin E supplementation resulted in higher expression of genes involved in cell cycle regulation (Ccnb2, Cdc2, Cdc6) in old T cells. Vitamin E supplementation resulted in higher up-regulation of IL-2 expression in young and old T cells and lower up-regulation of IL-4 expression in old T cells following stimulation. These findings suggest that aging has significant effects on the expression of genes associated with signal transduction, transcriptional regulation, and apoptosis pathways in T cells, and vitamin E has a significant impact on the expression of genes associated with cell cycle and Th1/Th2 balance in old T cells. Further studies are needed to determine whether these changes are due to the effects of aging at a single-cell level or to the shift in the ratio of naïve:memory T cells with age.

Original languageEnglish
Pages (from-to)6052-6061
Number of pages10
JournalJournal of Immunology
Volume177
Issue number9
Publication statusPublished - 2006 Nov 1

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Vitamin E
Transcriptome
T-Lymphocytes
Cell Cycle
Apoptosis
Gene Expression
Signal Transduction
Up-Regulation
Th1-Th2 Balance
Microarray Analysis
Inbred C57BL Mouse
Chemokines
Interleukin-4
Interleukin-2
Intercellular Signaling Peptides and Proteins
Cytokines
Diet

ASJC Scopus subject areas

  • Immunology

Cite this

Hau, S. N., Adolfsson, O., Lee, C-K., Prolla, T. A., Ordovas, J., & Meydani, S. N. (2006). Age- and vitamin E-induced changes in gene expression profiles of T cells. Journal of Immunology, 177(9), 6052-6061.

Age- and vitamin E-induced changes in gene expression profiles of T cells. / Hau, Sung Nim; Adolfsson, Oskar; Lee, Cheol-Koo; Prolla, Tomas A.; Ordovas, Jose; Meydani, Simin Nikbin.

In: Journal of Immunology, Vol. 177, No. 9, 01.11.2006, p. 6052-6061.

Research output: Contribution to journalArticle

Hau, SN, Adolfsson, O, Lee, C-K, Prolla, TA, Ordovas, J & Meydani, SN 2006, 'Age- and vitamin E-induced changes in gene expression profiles of T cells', Journal of Immunology, vol. 177, no. 9, pp. 6052-6061.
Hau SN, Adolfsson O, Lee C-K, Prolla TA, Ordovas J, Meydani SN. Age- and vitamin E-induced changes in gene expression profiles of T cells. Journal of Immunology. 2006 Nov 1;177(9):6052-6061.
Hau, Sung Nim ; Adolfsson, Oskar ; Lee, Cheol-Koo ; Prolla, Tomas A. ; Ordovas, Jose ; Meydani, Simin Nikbin. / Age- and vitamin E-induced changes in gene expression profiles of T cells. In: Journal of Immunology. 2006 ; Vol. 177, No. 9. pp. 6052-6061.
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