Aggregation-enhanced fluorescence in organically modified silica nanoparticles: A novel approach toward high-signal-output nanoprobes for two-photon fluorescence bioimaging

Sehoon Kim, Haridas E. Pudavar, Adela Bonoiu, Paras N. Prasad

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

A new two-photon fluorescent nanoprobe based on originally modified silica (ORMOSIL) nanoparticles loaded with high concentration of a dye exhibiting aggregation-enhanced two-photon adsorption (TPA) and fluorescence bioimaging was reported. The particle size was analyzed by transmission electron microscopic (TEM) images showing a good agreement with the number-averaged size distribution in aqueous suspension. The results show that after removal of the anoinic surfactant (AOT) by dialysis against deionized water, all the composite nanoparticles have a negative potential. The fluorescence spectrum of the composite nanoparticles are red-shifted with the increasing TPA dye loading, due to an increase in the effective π-conjugation length. The TPA dye in ORMOSIL nanoparticles has the potential to achieve several orders of magnitude improvement in the intracellular TPA signal, over existing fluorescent dye.

Original languageEnglish
Pages (from-to)3791-3795
Number of pages5
JournalAdvanced Materials
Volume19
Issue number22
DOIs
Publication statusPublished - 2007 Nov 19
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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