AGO1-miR173 complex initiates phased siRNA formation in plants

Taiowa A. Montgomery, Jeon Yoo Seong, Noah Fahlgren, Sunny D. Gilbert, Miya D. Howell, Christopher M. Sullivan, Amanda Alexander, Goretti Nguyen, Edwards Allen, Ji Hoon Ahn, James C. Carrington

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

MicroRNA (miRNA)-guided cleavage initiates entry of primary transcripts into the transacting siRNA (tasiRNA) biogenesis pathway involving RNA-DEPENDENT RNA POLYMERASE6, DICER-LIKE4, and SUPPRESSOR OF GENE SILENCING3. Arabidopsis thaliana TAS1 and TAS2 families yield tasiRNA that form through miR173-guided initiation-cleavage of primary transcripts and target several transcripts encoding pentatricopeptide repeat proteins and proteins of unknown function. Here, the TAS1c locus was modified to produce synthetic (syn) tasiRNA to target an endogenous transcript encoding PHYTOENE DESATURASE and used to analyze the role of miR173 in routing of transcripts through the tasiRNA pathway. miR173 was unique from other miRNAs in its ability to initiate TAS1c-based syn-tasiRNA formation. A single miR173 target site was sufficient to route non-TAS transcripts into the pathway to yield phased siRNA. We also show that miR173 functions in association with ARGONAUTE 1 (AGO1) during TAS1 and TAS2 tasiRNA formation, and we provide data indicating that the miR173-AGO1 complex possesses unique functionality that many other miRNA-AGO1 complexes lack.

Original languageEnglish
Pages (from-to)20055-20062
Number of pages8
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number51
DOIs
Publication statusPublished - 2008 Dec 23

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Small Interfering RNA
MicroRNAs
RNA
Suppressor Genes
Arabidopsis
Proteins

Keywords

  • Arabidopsis
  • ARGONAUTE
  • Microrna
  • Transacting sirna

ASJC Scopus subject areas

  • General

Cite this

Montgomery, T. A., Seong, J. Y., Fahlgren, N., Gilbert, S. D., Howell, M. D., Sullivan, C. M., ... Carrington, J. C. (2008). AGO1-miR173 complex initiates phased siRNA formation in plants. Proceedings of the National Academy of Sciences of the United States of America, 105(51), 20055-20062. https://doi.org/10.1073/pnas.0810241105

AGO1-miR173 complex initiates phased siRNA formation in plants. / Montgomery, Taiowa A.; Seong, Jeon Yoo; Fahlgren, Noah; Gilbert, Sunny D.; Howell, Miya D.; Sullivan, Christopher M.; Alexander, Amanda; Nguyen, Goretti; Allen, Edwards; Ahn, Ji Hoon; Carrington, James C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 51, 23.12.2008, p. 20055-20062.

Research output: Contribution to journalArticle

Montgomery, TA, Seong, JY, Fahlgren, N, Gilbert, SD, Howell, MD, Sullivan, CM, Alexander, A, Nguyen, G, Allen, E, Ahn, JH & Carrington, JC 2008, 'AGO1-miR173 complex initiates phased siRNA formation in plants', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 51, pp. 20055-20062. https://doi.org/10.1073/pnas.0810241105
Montgomery, Taiowa A. ; Seong, Jeon Yoo ; Fahlgren, Noah ; Gilbert, Sunny D. ; Howell, Miya D. ; Sullivan, Christopher M. ; Alexander, Amanda ; Nguyen, Goretti ; Allen, Edwards ; Ahn, Ji Hoon ; Carrington, James C. / AGO1-miR173 complex initiates phased siRNA formation in plants. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 51. pp. 20055-20062.
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