Agonists of the Mas-related gene (Mrgs) orphan receptors as novel mediators of mast cell-sensory nerve interactions

Min-Goo Lee, Xinzhong Dong, Qin Liu, Kush N. Patel, Oksoon Hong Choi, Becky Vonakis, Bradley J. Undem

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

IgE-dependent activation of mast cell activation is often associated with symptoms attributed to activation of sensory nerves. Depending on the tissues involved such symptoms include itching, sneezing, irritation, vasodilation, and reflex secretions. In the present study, we hypothesize that sensory neuroactive mediators released from mast cells may include agonists of recently discovered orphan receptors referred to as sensory nerve specific receptors or products of mas related genes. HEK-293 cells expressing MrgC11 receptors and wild-type HEK-293 cells were loaded with the calcium indicator Fura-2. A known stimulant of MrgC11 receptors the RF-amide, neuropeptide FF, evoked a rapid increase in cytosolic calcium in the MrgC11 expressing cells but not in the wild-type HEK-293 cells. IgE-dependent stimulation of either rat basophilic leukemia-2H3 cells (RBL-2H3 cells) or mouse bone marrow-derived mast cells, released a substance(s) that stimulated increases in cytosolic calcium in the MrgC11 expressing cells that far exceeded that seen in control cells. RT-PCR revealed that both mouse mast cells and RBL-2H3 cells express the RF-amide precursor gene proneuropeptide FF (A). Immunohistochemical analysis demonstrated RF-amide immunoreactivity in mouse skin mast cells in situ and in mast cells isolated from mouse skin. These data support the hypothesis that agonists of certain sensory nerve specific receptors or mas related genes may participate in mast cell sensory nerve interactions.

Original languageEnglish
Pages (from-to)2251-2255
Number of pages5
JournalJournal of Immunology
Volume180
Issue number4
DOIs
Publication statusPublished - 2008 Feb 15

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Mast Cells
HEK293 Cells
Genes
Amides
Calcium
Immunoglobulin E
Leukemia
Sneezing
Skin
Fura-2
Pruritus
Vasodilation
Reflex
Bone Marrow
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology

Cite this

Agonists of the Mas-related gene (Mrgs) orphan receptors as novel mediators of mast cell-sensory nerve interactions. / Lee, Min-Goo; Dong, Xinzhong; Liu, Qin; Patel, Kush N.; Choi, Oksoon Hong; Vonakis, Becky; Undem, Bradley J.

In: Journal of Immunology, Vol. 180, No. 4, 15.02.2008, p. 2251-2255.

Research output: Contribution to journalArticle

Lee, Min-Goo ; Dong, Xinzhong ; Liu, Qin ; Patel, Kush N. ; Choi, Oksoon Hong ; Vonakis, Becky ; Undem, Bradley J. / Agonists of the Mas-related gene (Mrgs) orphan receptors as novel mediators of mast cell-sensory nerve interactions. In: Journal of Immunology. 2008 ; Vol. 180, No. 4. pp. 2251-2255.
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