AIMP1 deficiency enhances airway hyperreactivity in mice via increased T H2 immune responses

Hye Jin Hong, Eugene Kim, Mi Young Jung, Sunghoon Kim, Tae Sung Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aminoacyl tRNA synthetase complex-interacting multicomplex protein 1 (AIMP1) is known as a novel cytokine carrying out a variety of biological activities, including angiogenesis and wound repair. In our previous reports AIMP1 was demonstrated to induce T H1 polarization. However, the effects of AIMP1 deficiency in T H1 or T H2 immune disorders remain unclear. In this study, we characterized phenotypes of AIMP1-deficient mice and investigated the role of AIMP1 in T H2-biased airway hyperreactivity. Clinical signs of allergic airway inflammation were assessed in AIMP1-deficient mice and the effects of AIMP1 deficiency on production of T H2 cytokines were evaluated in T cells using AIMP1-specific siRNA. Additionally, the enhanced pause values and histologic analysis were assessed in mice receiving AIMP1-deficient CD4 + T cells with OVA challenge. Clinical signs of spontaneous airway inflammation were noted in AIMP1-deficienct mice. AIMP1-deficient mice showed strongly increased Penh values in response to methacholine without any allergen exposure. Adoptive transfer of AIMP1-deficient CD4 + T cells to OVA-sensitized C57BL/6 mice exacerbated OVA-induced airway inflammation and increased infiltration of inflammatory cells into the lung. Furthermore, lung DCs in AIMP1-deficient mice showed increased expression of surface molecules, and IL-12p40 level in sera significantly decreased in AIMP1-deficient mice compared to that of wild type mice. These results strongly indicate that AIMP1 plays a role in negatively regulating T H2 responses in vivo, and AIMP1 can be employed as a novel therapeutic agent against T H2-biased diseases, particularly asthma.

Original languageEnglish
Pages (from-to)256-265
Number of pages10
JournalClinical Immunology
Volume143
Issue number3
DOIs
Publication statusPublished - 2012 Jun 1

Fingerprint

Inflammation
T-Lymphocytes
Interleukin-12 Subunit p40
Cytokines
Amino Acyl-tRNA Synthetases
Lung
Adoptive Transfer
Methacholine Chloride
Immune System Diseases
Inbred C57BL Mouse
Allergens
Small Interfering RNA
Asthma
Phenotype
Wounds and Injuries
Serum
Proteins
Therapeutics

Keywords

  • AIMP1
  • Asthma
  • CD4
  • Dendritic cell
  • T 2 response

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

AIMP1 deficiency enhances airway hyperreactivity in mice via increased T H2 immune responses. / Hong, Hye Jin; Kim, Eugene; Jung, Mi Young; Kim, Sunghoon; Kim, Tae Sung.

In: Clinical Immunology, Vol. 143, No. 3, 01.06.2012, p. 256-265.

Research output: Contribution to journalArticle

Hong, Hye Jin ; Kim, Eugene ; Jung, Mi Young ; Kim, Sunghoon ; Kim, Tae Sung. / AIMP1 deficiency enhances airway hyperreactivity in mice via increased T H2 immune responses. In: Clinical Immunology. 2012 ; Vol. 143, No. 3. pp. 256-265.
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