AIMP1 regulates TCR signaling and induces differentiation of regulatory T cells by interfering with lipid raft association

Myun Soo Kim, Arim Lee, Dae Ho Cho, Tae Sung Kim

Research output: Contribution to journalArticle

Abstract

In addition to a role in translation, AIMP1 is secreted to affect various immune cells, such as macrophages, dendritic cells, B cells, and natural killer cells. However, the direct effects of AIMP1 on T cells have not yet been reported. In this study, we investigated whether AIMP1 could modulate T cell responses directly. Results revealed that AIMP1 significantly inhibited T cell receptor (TCR)-dependent activation and proliferation of CD4 T cells, as well as decreased TCR stimuli-induced Ca 2+ influx in CD4 T cells. In addition, microscopic analysis revealed that lipid raft association in response to TCR engagement was significantly reduced in the presence of AIMP1, and the phosphorylation of PLCγ and PI3K was also down-regulated in CD4 T cells by AIMP1. Furthermore, AIMP1 specifically enhanced the differentiation of regulatory T (Treg)cells, while it had no effect on T helper type 1 (Th1), type 2 (Th2), and type 17 (Th17)cell differentiation. Collectively, these results indicate that AIMP1 affects T cells directly by down-regulating TCR signaling complex formation and inducing Treg cell differentiation in CD4 T cells.

Original languageEnglish
JournalBiochemical and biophysical research communications
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

T-cells
Regulatory T-Lymphocytes
T-Cell Antigen Receptor
Association reactions
T-Lymphocytes
Lipids
Cell Differentiation
Phosphorylation
Macrophages
Programmable logic controllers
Phosphatidylinositol 3-Kinases
Natural Killer Cells
Dendritic Cells
B-Lymphocytes
Chemical activation
Cells

Keywords

  • AIMP1
  • Calcium flux
  • Lipid raft
  • TCR signal

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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title = "AIMP1 regulates TCR signaling and induces differentiation of regulatory T cells by interfering with lipid raft association",
abstract = "In addition to a role in translation, AIMP1 is secreted to affect various immune cells, such as macrophages, dendritic cells, B cells, and natural killer cells. However, the direct effects of AIMP1 on T cells have not yet been reported. In this study, we investigated whether AIMP1 could modulate T cell responses directly. Results revealed that AIMP1 significantly inhibited T cell receptor (TCR)-dependent activation and proliferation of CD4 T cells, as well as decreased TCR stimuli-induced Ca 2+ influx in CD4 T cells. In addition, microscopic analysis revealed that lipid raft association in response to TCR engagement was significantly reduced in the presence of AIMP1, and the phosphorylation of PLCγ and PI3K was also down-regulated in CD4 T cells by AIMP1. Furthermore, AIMP1 specifically enhanced the differentiation of regulatory T (Treg)cells, while it had no effect on T helper type 1 (Th1), type 2 (Th2), and type 17 (Th17)cell differentiation. Collectively, these results indicate that AIMP1 affects T cells directly by down-regulating TCR signaling complex formation and inducing Treg cell differentiation in CD4 T cells.",
keywords = "AIMP1, Calcium flux, Lipid raft, TCR signal",
author = "Kim, {Myun Soo} and Arim Lee and Cho, {Dae Ho} and Kim, {Tae Sung}",
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AU - Kim, Myun Soo

AU - Lee, Arim

AU - Cho, Dae Ho

AU - Kim, Tae Sung

PY - 2019/1/1

Y1 - 2019/1/1

N2 - In addition to a role in translation, AIMP1 is secreted to affect various immune cells, such as macrophages, dendritic cells, B cells, and natural killer cells. However, the direct effects of AIMP1 on T cells have not yet been reported. In this study, we investigated whether AIMP1 could modulate T cell responses directly. Results revealed that AIMP1 significantly inhibited T cell receptor (TCR)-dependent activation and proliferation of CD4 T cells, as well as decreased TCR stimuli-induced Ca 2+ influx in CD4 T cells. In addition, microscopic analysis revealed that lipid raft association in response to TCR engagement was significantly reduced in the presence of AIMP1, and the phosphorylation of PLCγ and PI3K was also down-regulated in CD4 T cells by AIMP1. Furthermore, AIMP1 specifically enhanced the differentiation of regulatory T (Treg)cells, while it had no effect on T helper type 1 (Th1), type 2 (Th2), and type 17 (Th17)cell differentiation. Collectively, these results indicate that AIMP1 affects T cells directly by down-regulating TCR signaling complex formation and inducing Treg cell differentiation in CD4 T cells.

AB - In addition to a role in translation, AIMP1 is secreted to affect various immune cells, such as macrophages, dendritic cells, B cells, and natural killer cells. However, the direct effects of AIMP1 on T cells have not yet been reported. In this study, we investigated whether AIMP1 could modulate T cell responses directly. Results revealed that AIMP1 significantly inhibited T cell receptor (TCR)-dependent activation and proliferation of CD4 T cells, as well as decreased TCR stimuli-induced Ca 2+ influx in CD4 T cells. In addition, microscopic analysis revealed that lipid raft association in response to TCR engagement was significantly reduced in the presence of AIMP1, and the phosphorylation of PLCγ and PI3K was also down-regulated in CD4 T cells by AIMP1. Furthermore, AIMP1 specifically enhanced the differentiation of regulatory T (Treg)cells, while it had no effect on T helper type 1 (Th1), type 2 (Th2), and type 17 (Th17)cell differentiation. Collectively, these results indicate that AIMP1 affects T cells directly by down-regulating TCR signaling complex formation and inducing Treg cell differentiation in CD4 T cells.

KW - AIMP1

KW - Calcium flux

KW - Lipid raft

KW - TCR signal

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