AIMP1/p43 protein induces the maturation of bone marrow-derived dendritic cells with T helper type 1-polarizing ability

Eugene Kim, Hyun Kim Seung, Sunghoon Kim, Dae Ho Cho, Tae Sung Kim

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33 Citations (Scopus)

Abstract

AIMP1 (ARS-interacting multifunctional protein 1) , previously known as p43, was initially identified as a factor associated with a macromolecular tRNA synthetase complex. Recently, we demonstrated that AIMP1 is also secreted and acts as a novel pleiotropic cytokine. In this study, we investigated whether AIMP1 induces the activation and maturation of murine bone marrow-derived dendritic cells (DCs). AIMP1-treated DCs exhibited up-regulated expression of cell-surface molecules, including CD40, CD86, and MHC class II. Additionally, microarray analysis and RT-PCR determinations indicated that the expression of known DC maturation genes also increased significantly following treatment with AIMP1. Treatment of DCs with AIMP1 resulted in a significant increase in IL-12 production and Ag-presenting capability, and it also stimulated the proliferation of allogeneic T cells. Importantly, AIMP1-treated DCs induced activation of Ag-specific Th type 1 (Th1) cells in vitro and in vivo. AIMP1-stimulated DCs significantly enhanced the IFN-γ production of cocultured CD4+ T cells. Immunization of mice with keyhole limpet hemocyanin-pulsed AIMP1 DCs efficiently led to Ag-specific Th1 cell responses, as determined by flow cytometry and ELISA. The addition of a neutralizing anti-IL-12 mAb to the cell cultures that had been treated with AIMP1 resulted in the decreased production of IFN-γ, thereby indicating that AIMP1-stimulated DCs may enhance the Th1 response through increased production of IL-12 by APCs. Taken together, these results indicate that AIMP1 protein induces the maturation and activation of DCs, which skew the immune response toward a Th1 response.

Original languageEnglish
Pages (from-to)2894-2902
Number of pages9
JournalJournal of Immunology
Volume180
Issue number5
Publication statusPublished - 2008 Mar 1

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Dendritic Cells
Bone Marrow
Proteins
Interleukin-12
T-Lymphocytes
Amino Acyl-tRNA Synthetases
Microarray Analysis
Immunization
Flow Cytometry
Cell Culture Techniques
Enzyme-Linked Immunosorbent Assay
Cytokines
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology

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AIMP1/p43 protein induces the maturation of bone marrow-derived dendritic cells with T helper type 1-polarizing ability. / Kim, Eugene; Seung, Hyun Kim; Kim, Sunghoon; Cho, Dae Ho; Kim, Tae Sung.

In: Journal of Immunology, Vol. 180, No. 5, 01.03.2008, p. 2894-2902.

Research output: Contribution to journalArticle

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