Albumin expression is required for adipocyte differentiation of 3T3-L1 cells

Wonbaek Yoo, Jaeseob Lee, Sangeun Park, Young Sik Kim, Chae Seung Lim, Eul Sik Yoon, Gangmin Hur, Jun Seo Oh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have previously demonstrated that albumin is directly involved in the formation of cytoplasmic lipid droplets in pancreatic stellate cells and may act as a downstream effector of adipogenic transcription factors, PPAR-γ and C/EBP-α. Here, we investigated the role of albumin in adipocyte differentiation using 3T3-L1 cells. Albumin expression was significantly increased at later stages of adipocyte differentiation, which was accompanied with increased C/EBP-β binding to albumin promoter. Suppression of albumin expression using short-hairpin RNA (shRNA) during differentiation led to a considerable reduction in lipid droplet formation, whereas albumin overexpression was stimulatory. Furthermore, point mutation in its fatty acid-binding sites inhibited lipid droplet formation. Consistent with these in vitro finding, Nagase analbuminemic rats displayed reduced fat accumulation. Therefore, our findings suggest that albumin may play a distinct role in adipocyte differentiation by promoting lipid accumulation.

Original languageEnglish
Pages (from-to)170-175
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume397
Issue number2
DOIs
Publication statusPublished - 2010 Jun 25

Keywords

  • 3T3-L1 adipocyte
  • Adipogenesis
  • Albumin
  • C/EBP-α
  • Lipid droplet formation
  • PPAR-γ

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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