Albumin expression is required for adipocyte differentiation of 3T3-L1 cells

Wonbaek Yoo, Jaeseob Lee, Sangeun Park, Young Sik Kim, Chaeseung Lim, Eulsik Yoon, Gangmin Hur, Junseo Oh

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We have previously demonstrated that albumin is directly involved in the formation of cytoplasmic lipid droplets in pancreatic stellate cells and may act as a downstream effector of adipogenic transcription factors, PPAR-γ and C/EBP-α. Here, we investigated the role of albumin in adipocyte differentiation using 3T3-L1 cells. Albumin expression was significantly increased at later stages of adipocyte differentiation, which was accompanied with increased C/EBP-β binding to albumin promoter. Suppression of albumin expression using short-hairpin RNA (shRNA) during differentiation led to a considerable reduction in lipid droplet formation, whereas albumin overexpression was stimulatory. Furthermore, point mutation in its fatty acid-binding sites inhibited lipid droplet formation. Consistent with these in vitro finding, Nagase analbuminemic rats displayed reduced fat accumulation. Therefore, our findings suggest that albumin may play a distinct role in adipocyte differentiation by promoting lipid accumulation.

Original languageEnglish
Pages (from-to)170-175
Number of pages6
JournalBiochemical and biophysical research communications
Volume397
Issue number2
DOIs
Publication statusPublished - 2010 Jun 25

Keywords

  • 3T3-L1 adipocyte
  • Adipogenesis
  • Albumin
  • C/EBP-α
  • Lipid droplet formation
  • PPAR-γ

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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