Albumin mediates PPAR-γ or C/EBP-α-induced phenotypic changes in pancreatic stellate cells

Nayoung Kim, Soyoung Choi, Chae Seung Lim, Hong Sik Lee, Jun Seo Oh

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Activation of quiescent hepatic stellate cells (HSCs) into myofibroblast-like cells is a key event of liver fibrosis, and adipogenic transcription factors, PPAR-γ and C/EBP-α, reverse HSC activation. As albumin was reported to maintain the quiescent phenotype of stellate cells, we examined whether it plays a role in PPAR-γ and C/EBP-α-mediated effects. Pancreatic stellate cells (PSCs) were isolated from rat pancreas and used in their culture-activated phenotype. Forced expression of PPAR-γ or C/EBP-α in PSCs increased albumin mRNA and protein levels by >2.5-fold, which is accompanied with increased C/EBP-β binding to albumin promoter. PPAR-γ and C/EBP-α also induced a phenotypic switch from activated to quiescent cells and, interestingly, suppression of albumin using short-hairpin RNA (shRNA) blocked their effects. Therefore, our findings suggest that albumin may be a downstream effector of PPAR-γ and C/EBP-α in PSCs and that it can be an attractive molecular target for anti-fibrotic therapies.

Original languageEnglish
Pages (from-to)640-644
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume391
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1

Fingerprint

Pancreatic Stellate Cells
Peroxisome Proliferator-Activated Receptors
Albumins
Hepatic Stellate Cells
Chemical activation
Phenotype
Myofibroblasts
Liver Cirrhosis
Liver
Small Interfering RNA
Rats
Pancreas
Transcription Factors
Switches
Messenger RNA
Proteins

Keywords

  • Albumin
  • Fibrosis
  • Lipid droplet formation
  • Pancreatic stellate cells
  • PPAR-γ

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Albumin mediates PPAR-γ or C/EBP-α-induced phenotypic changes in pancreatic stellate cells. / Kim, Nayoung; Choi, Soyoung; Lim, Chae Seung; Lee, Hong Sik; Oh, Jun Seo.

In: Biochemical and Biophysical Research Communications, Vol. 391, No. 1, 01.01.2010, p. 640-644.

Research output: Contribution to journalArticle

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