Aldosterone modulates cell proliferation and apoptosis in the neonatal rat heart

Hyung Joo Sohn, Kee Hwan Yoo, Gi Young Jang, Jang Hoon Lee, Byung Min Choi, Jung Hwa Lee, In Sun Bae, Hyung Eun Yim, Chang Sung Son, Joo Won Lee

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11 Citations (Scopus)

Abstract

In the present study, we investigated whether and how the mineralocorticoid receptor antagonist spironolactone affects cardiac growth and development through apoptosis and cell proliferation in the neonatal rat heart. Newborn rat pups were treated with spironolactone (200 mg/kg/d) for 7 days. The cell proliferation was studied by PCNA immunostaining. The treatment with spironolactone decreased proliferating myocytes by 32% (P<0.05), and reduced myocytes apoptosis by 29% (P<0.05). Immunoblot and immunohistochemistry for the expression of p38, p53, clusterin, TGF-β2, and extracellular signal-regulated kinase were performed. In the spironolactone group, p38, p53, clusterin, and TGF-β2 protein expression was significantly decreased (P<0.05). These results indicate that aldosterone inhibition in the developing rat heart induces cardiac growth impairment by decreasing proliferation and apoptosis of myocytes.

Original languageEnglish
Pages (from-to)1296-1304
Number of pages9
JournalJournal of Korean medical science
Volume25
Issue number9
DOIs
Publication statusPublished - 2010 Sep

Keywords

  • Aldosterone
  • Apoptosis
  • Cell proliferation
  • Muscle cells

ASJC Scopus subject areas

  • Medicine(all)

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    Sohn, H. J., Yoo, K. H., Jang, G. Y., Lee, J. H., Choi, B. M., Lee, J. H., Bae, I. S., Yim, H. E., Son, C. S., & Lee, J. W. (2010). Aldosterone modulates cell proliferation and apoptosis in the neonatal rat heart. Journal of Korean medical science, 25(9), 1296-1304. https://doi.org/10.3346/jkms.2010.25.9.1296