Abstract
Growing evidence indicates that aldosterone is a potent mitogenic signal regulating genes involved in antiapoptosis, cell proliferation and growth. We investigated the role of endogenous aldosterone in renal development, cell proliferation and apoptosis, and mitogen-activated protein kinase (MAPK) family expression. Newborn rats were treated with either spironolactone (200 mg/kg/d) in olive oil or only olive oil for 7 days. TUNEL assay and proliferating cell nuclear antigen (PCNA) stain were performed on kidney sections. Immunoblots, immunohistochemical (IHC) stain, and reverse transcriptase-PCR for MAPKs were performed. PCNA-positive proliferating cells decreased and apoptotic cells increased significantly with spironolactone (P<0.05). In the spironolactone-treated group, c-jun N-terminal kinase (JNK)-2 expression increased, whereas extracellular signal regulated kinase (ERK)-2 and p38 expressions decreased in immunoblots (P<0.05) and IHC stain. ERK-2 and p38 mRNA expressions increased in the spironolactone-treated group (P<0.05). This study demonstrates that aldosterone blockade in the developing kidney decreases cellular proliferation, increases apoptosis, and modulates the expressions of JNK-2, ERK-2, and p38. Aldosterone possibly participates in renal development and MAPK family may serve as, in part, the signaling intermediate through the mineralocorticoid receptor (MR) in the developing kidney.
| Original language | English |
|---|---|
| Pages (from-to) | 724-733 |
| Number of pages | 10 |
| Journal | Journal of Cellular Physiology |
| Volume | 219 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2009 Jun 1 |
Fingerprint
ASJC Scopus subject areas
- Physiology
- Clinical Biochemistry
- Cell Biology
Cite this
Aldosterone regulates cellular turnover and mitogen-activated protein kinase family expression in the neonatal rat kidney. / Yim, Hyung-Eun; Yoo, Kee Hwan; In, Sun Bae; Jang, Giyoung; Hong, Young Sook; Joo, Won Lee.
In: Journal of Cellular Physiology, Vol. 219, No. 3, 01.06.2009, p. 724-733.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aldosterone regulates cellular turnover and mitogen-activated protein kinase family expression in the neonatal rat kidney
AU - Yim, Hyung-Eun
AU - Yoo, Kee Hwan
AU - In, Sun Bae
AU - Jang, Giyoung
AU - Hong, Young Sook
AU - Joo, Won Lee
PY - 2009/6/1
Y1 - 2009/6/1
N2 - Growing evidence indicates that aldosterone is a potent mitogenic signal regulating genes involved in antiapoptosis, cell proliferation and growth. We investigated the role of endogenous aldosterone in renal development, cell proliferation and apoptosis, and mitogen-activated protein kinase (MAPK) family expression. Newborn rats were treated with either spironolactone (200 mg/kg/d) in olive oil or only olive oil for 7 days. TUNEL assay and proliferating cell nuclear antigen (PCNA) stain were performed on kidney sections. Immunoblots, immunohistochemical (IHC) stain, and reverse transcriptase-PCR for MAPKs were performed. PCNA-positive proliferating cells decreased and apoptotic cells increased significantly with spironolactone (P<0.05). In the spironolactone-treated group, c-jun N-terminal kinase (JNK)-2 expression increased, whereas extracellular signal regulated kinase (ERK)-2 and p38 expressions decreased in immunoblots (P<0.05) and IHC stain. ERK-2 and p38 mRNA expressions increased in the spironolactone-treated group (P<0.05). This study demonstrates that aldosterone blockade in the developing kidney decreases cellular proliferation, increases apoptosis, and modulates the expressions of JNK-2, ERK-2, and p38. Aldosterone possibly participates in renal development and MAPK family may serve as, in part, the signaling intermediate through the mineralocorticoid receptor (MR) in the developing kidney.
AB - Growing evidence indicates that aldosterone is a potent mitogenic signal regulating genes involved in antiapoptosis, cell proliferation and growth. We investigated the role of endogenous aldosterone in renal development, cell proliferation and apoptosis, and mitogen-activated protein kinase (MAPK) family expression. Newborn rats were treated with either spironolactone (200 mg/kg/d) in olive oil or only olive oil for 7 days. TUNEL assay and proliferating cell nuclear antigen (PCNA) stain were performed on kidney sections. Immunoblots, immunohistochemical (IHC) stain, and reverse transcriptase-PCR for MAPKs were performed. PCNA-positive proliferating cells decreased and apoptotic cells increased significantly with spironolactone (P<0.05). In the spironolactone-treated group, c-jun N-terminal kinase (JNK)-2 expression increased, whereas extracellular signal regulated kinase (ERK)-2 and p38 expressions decreased in immunoblots (P<0.05) and IHC stain. ERK-2 and p38 mRNA expressions increased in the spironolactone-treated group (P<0.05). This study demonstrates that aldosterone blockade in the developing kidney decreases cellular proliferation, increases apoptosis, and modulates the expressions of JNK-2, ERK-2, and p38. Aldosterone possibly participates in renal development and MAPK family may serve as, in part, the signaling intermediate through the mineralocorticoid receptor (MR) in the developing kidney.
UR - http://www.scopus.com/inward/record.url?scp=64549089670&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=64549089670&partnerID=8YFLogxK
U2 - 10.1002/jcp.21723
DO - 10.1002/jcp.21723
M3 - Article
VL - 219
SP - 724
EP - 733
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
IS - 3
ER -