Aldosterone regulates cellular turnover and mitogen-activated protein kinase family expression in the neonatal rat kidney

Hyung-Eun Yim, Kee Hwan Yoo, Sun Bae In, Giyoung Jang, Young Sook Hong, Won Lee Joo

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Growing evidence indicates that aldosterone is a potent mitogenic signal regulating genes involved in antiapoptosis, cell proliferation and growth. We investigated the role of endogenous aldosterone in renal development, cell proliferation and apoptosis, and mitogen-activated protein kinase (MAPK) family expression. Newborn rats were treated with either spironolactone (200 mg/kg/d) in olive oil or only olive oil for 7 days. TUNEL assay and proliferating cell nuclear antigen (PCNA) stain were performed on kidney sections. Immunoblots, immunohistochemical (IHC) stain, and reverse transcriptase-PCR for MAPKs were performed. PCNA-positive proliferating cells decreased and apoptotic cells increased significantly with spironolactone (P<0.05). In the spironolactone-treated group, c-jun N-terminal kinase (JNK)-2 expression increased, whereas extracellular signal regulated kinase (ERK)-2 and p38 expressions decreased in immunoblots (P<0.05) and IHC stain. ERK-2 and p38 mRNA expressions increased in the spironolactone-treated group (P<0.05). This study demonstrates that aldosterone blockade in the developing kidney decreases cellular proliferation, increases apoptosis, and modulates the expressions of JNK-2, ERK-2, and p38. Aldosterone possibly participates in renal development and MAPK family may serve as, in part, the signaling intermediate through the mineralocorticoid receptor (MR) in the developing kidney.

Original languageEnglish
Pages (from-to)724-733
Number of pages10
JournalJournal of Cellular Physiology
Volume219
Issue number3
DOIs
Publication statusPublished - 2009 Jun 1

Fingerprint

Spironolactone
Mitogen-Activated Protein Kinases
Aldosterone
Rats
Mitogen-Activated Protein Kinase 1
Kidney
Coloring Agents
Proliferating Cell Nuclear Antigen
Cell proliferation
Cell Proliferation
Mitogen-Activated Protein Kinase 9
Apoptosis
Mineralocorticoid Receptors
RNA-Directed DNA Polymerase
Cell growth
In Situ Nick-End Labeling
Assays
Reverse Transcriptase Polymerase Chain Reaction
Genes
Cells

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Aldosterone regulates cellular turnover and mitogen-activated protein kinase family expression in the neonatal rat kidney. / Yim, Hyung-Eun; Yoo, Kee Hwan; In, Sun Bae; Jang, Giyoung; Hong, Young Sook; Joo, Won Lee.

In: Journal of Cellular Physiology, Vol. 219, No. 3, 01.06.2009, p. 724-733.

Research output: Contribution to journalArticle

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