Allium sativum potentiates suicide gene therapy for murine transitional cell carcinoma

D. G. Moon, J. Cheon, D. H. Yoon, H. S. Park, Han Kyeom Kim, J. J. Kim, S. K. Koh

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    This study evaluated the synergistic effect of Allium sativum (AS) with suicide gene therapy for transitional cell carcinoma (TCC) of the bladder. Subcutaneous TCCs were established in syngeneic C3H/He mice with 1 × 105 MBT-2 cells. AS liquid extract was injected at the site of tumor transplantation on Day 1 for three weeks (Experiment I) and into the established tumors weekly for five weeks (Experiment II) in combination with or without gene therapy using a replication-defective adenoviral vector containing a herpes simplex virus thymidine kinase (HSV-TK) gene under the transcriptional control of Rous sarcoma virus (RSV) promoter (Ad-RSV-TK, 5 × 108 plaque-forming units) plus ganciclovir (20 mg/kg/day ip). AS demonstrated a statistically significant reduction in incidence of TCC (cumulative dose 25 mg of AS). Combination AS-suicide gene therapy significantly inhibited the tumor growth compared with the controls, which was evidenced by apoptosis on histomorphological and immunohistochemical studies. These results suggest that AS had a definite antitumor effect in inhibiting tumorigenesis and growth of TCC in a murine model. AS treatment combined with suicide gene therapy had significant additive antitumor effects on TCC and may provide a novel and effective treatment modality for TCC of the bladder.

    Original languageEnglish
    Pages (from-to)98-105
    Number of pages8
    JournalNutrition and Cancer
    Volume38
    Issue number1
    DOIs
    Publication statusPublished - 2000

    ASJC Scopus subject areas

    • Medicine (miscellaneous)
    • Oncology
    • Nutrition and Dietetics
    • Cancer Research

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