Allium sativum potentiates suicide gene therapy for murine transitional cell carcinoma

Du Geon Moon, Jun Cheon, D. H. Yoon, Hong Seok Park, H. K. Kim, Je-Jong Kim, S. K. Koh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study evaluated the synergistic effect of Allium sativum (AS) with suicide gene therapy for transitional cell carcinoma (TCC) of the bladder. Subcutaneous TCCs were established in syngeneic C3H/He mice with 1 × 105 MBT-2 cells. AS liquid extract was injected at the site of tumor transplantation on Day 1 for three weeks (Experiment I) and into the established tumors weekly for five weeks (Experiment II) in combination with or without gene therapy using a replication-defective adenoviral vector containing a herpes simplex virus thymidine kinase (HSV-TK) gene under the transcriptional control of Rous sarcoma virus (RSV) promoter (Ad-RSV-TK, 5 × 108 plaque-forming units) plus ganciclovir (20 mg/kg/day ip). AS demonstrated a statistically significant reduction in incidence of TCC (cumulative dose 25 mg of AS). Combination AS-suicide gene therapy significantly inhibited the tumor growth compared with the controls, which was evidenced by apoptosis on histomorphological and immunohistochemical studies. These results suggest that AS had a definite antitumor effect in inhibiting tumorigenesis and growth of TCC in a murine model. AS treatment combined with suicide gene therapy had significant additive antitumor effects on TCC and may provide a novel and effective treatment modality for TCC of the bladder.

Original languageEnglish
Pages (from-to)98-105
Number of pages8
JournalNutrition and Cancer
Volume38
Issue number1
DOIs
Publication statusPublished - 2000 Jan 1

Fingerprint

Garlic
Transitional Cell Carcinoma
Genetic Therapy
Suicide
Rous sarcoma virus
Urinary Bladder
Neoplasms
Ganciclovir
Thymidine Kinase
Inbred C3H Mouse
Simplexvirus
Growth
Carcinogenesis
Transplantation
Apoptosis
Incidence
Therapeutics
Genes

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Oncology
  • Nutrition and Dietetics
  • Cancer Research

Cite this

Allium sativum potentiates suicide gene therapy for murine transitional cell carcinoma. / Moon, Du Geon; Cheon, Jun; Yoon, D. H.; Park, Hong Seok; Kim, H. K.; Kim, Je-Jong; Koh, S. K.

In: Nutrition and Cancer, Vol. 38, No. 1, 01.01.2000, p. 98-105.

Research output: Contribution to journalArticle

Moon, Du Geon ; Cheon, Jun ; Yoon, D. H. ; Park, Hong Seok ; Kim, H. K. ; Kim, Je-Jong ; Koh, S. K. / Allium sativum potentiates suicide gene therapy for murine transitional cell carcinoma. In: Nutrition and Cancer. 2000 ; Vol. 38, No. 1. pp. 98-105.
@article{47791f93cfb046859f228a279de4e706,
title = "Allium sativum potentiates suicide gene therapy for murine transitional cell carcinoma",
abstract = "This study evaluated the synergistic effect of Allium sativum (AS) with suicide gene therapy for transitional cell carcinoma (TCC) of the bladder. Subcutaneous TCCs were established in syngeneic C3H/He mice with 1 × 105 MBT-2 cells. AS liquid extract was injected at the site of tumor transplantation on Day 1 for three weeks (Experiment I) and into the established tumors weekly for five weeks (Experiment II) in combination with or without gene therapy using a replication-defective adenoviral vector containing a herpes simplex virus thymidine kinase (HSV-TK) gene under the transcriptional control of Rous sarcoma virus (RSV) promoter (Ad-RSV-TK, 5 × 108 plaque-forming units) plus ganciclovir (20 mg/kg/day ip). AS demonstrated a statistically significant reduction in incidence of TCC (cumulative dose 25 mg of AS). Combination AS-suicide gene therapy significantly inhibited the tumor growth compared with the controls, which was evidenced by apoptosis on histomorphological and immunohistochemical studies. These results suggest that AS had a definite antitumor effect in inhibiting tumorigenesis and growth of TCC in a murine model. AS treatment combined with suicide gene therapy had significant additive antitumor effects on TCC and may provide a novel and effective treatment modality for TCC of the bladder.",
author = "Moon, {Du Geon} and Jun Cheon and Yoon, {D. H.} and Park, {Hong Seok} and Kim, {H. K.} and Je-Jong Kim and Koh, {S. K.}",
year = "2000",
month = "1",
day = "1",
doi = "10.1207/S15327914NC381_14",
language = "English",
volume = "38",
pages = "98--105",
journal = "Nutrition and Cancer",
issn = "0163-5581",
publisher = "Routledge",
number = "1",

}

TY - JOUR

T1 - Allium sativum potentiates suicide gene therapy for murine transitional cell carcinoma

AU - Moon, Du Geon

AU - Cheon, Jun

AU - Yoon, D. H.

AU - Park, Hong Seok

AU - Kim, H. K.

AU - Kim, Je-Jong

AU - Koh, S. K.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - This study evaluated the synergistic effect of Allium sativum (AS) with suicide gene therapy for transitional cell carcinoma (TCC) of the bladder. Subcutaneous TCCs were established in syngeneic C3H/He mice with 1 × 105 MBT-2 cells. AS liquid extract was injected at the site of tumor transplantation on Day 1 for three weeks (Experiment I) and into the established tumors weekly for five weeks (Experiment II) in combination with or without gene therapy using a replication-defective adenoviral vector containing a herpes simplex virus thymidine kinase (HSV-TK) gene under the transcriptional control of Rous sarcoma virus (RSV) promoter (Ad-RSV-TK, 5 × 108 plaque-forming units) plus ganciclovir (20 mg/kg/day ip). AS demonstrated a statistically significant reduction in incidence of TCC (cumulative dose 25 mg of AS). Combination AS-suicide gene therapy significantly inhibited the tumor growth compared with the controls, which was evidenced by apoptosis on histomorphological and immunohistochemical studies. These results suggest that AS had a definite antitumor effect in inhibiting tumorigenesis and growth of TCC in a murine model. AS treatment combined with suicide gene therapy had significant additive antitumor effects on TCC and may provide a novel and effective treatment modality for TCC of the bladder.

AB - This study evaluated the synergistic effect of Allium sativum (AS) with suicide gene therapy for transitional cell carcinoma (TCC) of the bladder. Subcutaneous TCCs were established in syngeneic C3H/He mice with 1 × 105 MBT-2 cells. AS liquid extract was injected at the site of tumor transplantation on Day 1 for three weeks (Experiment I) and into the established tumors weekly for five weeks (Experiment II) in combination with or without gene therapy using a replication-defective adenoviral vector containing a herpes simplex virus thymidine kinase (HSV-TK) gene under the transcriptional control of Rous sarcoma virus (RSV) promoter (Ad-RSV-TK, 5 × 108 plaque-forming units) plus ganciclovir (20 mg/kg/day ip). AS demonstrated a statistically significant reduction in incidence of TCC (cumulative dose 25 mg of AS). Combination AS-suicide gene therapy significantly inhibited the tumor growth compared with the controls, which was evidenced by apoptosis on histomorphological and immunohistochemical studies. These results suggest that AS had a definite antitumor effect in inhibiting tumorigenesis and growth of TCC in a murine model. AS treatment combined with suicide gene therapy had significant additive antitumor effects on TCC and may provide a novel and effective treatment modality for TCC of the bladder.

UR - http://www.scopus.com/inward/record.url?scp=0034446470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034446470&partnerID=8YFLogxK

U2 - 10.1207/S15327914NC381_14

DO - 10.1207/S15327914NC381_14

M3 - Article

C2 - 11341051

AN - SCOPUS:0034446470

VL - 38

SP - 98

EP - 105

JO - Nutrition and Cancer

JF - Nutrition and Cancer

SN - 0163-5581

IS - 1

ER -