Alpha-lipoic acid attenuates cisplatin-induced tubulointerstitial injuries through inhibition of mitochondrial bax translocation in rats

Young Mo Lee, So Yon Bae, Nam Hee Won, Heui Jung Pyo, Young-Joo Kwon

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Renal tubule cell apoptosis plays a pivotal role in cisplatin-induced nephrotoxicity. α-Lipoic acid (LA), a thiol antioxidant, is well known to be cytoprotective in various cell death models through its involvement in the death receptor apoptosis pathway. However, we hypothesized that LA would attenuate cisplatin-induced nephrotoxicity through inhibition of mitochondrial bax translocation in rats. Methods and Materials: Sprague-Dawley rats were treated with cisplatin (7 mg/kg) with or without pretreatment with LA (100 mg/kg × 3 times). Renal function was evaluated based on blood urea nitrogen (BUN), serum creatinine, and fractional excretion of sodium. Tubular necrosis scores were assessed by light microscopy findings and apoptotic cell deaths by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. Cytosolic bax, mitochondrial bax, cytochrome c, caspase-9 and caspase-3 were investigated using Western blot in each group. Results: LA pretreatment significantly decreased both BUN and serum creatinine. Morphologically, both tubular necrosis and apoptosis of tubular cells were decreased significantly with LA pretreatment. LA attenuated the translocation of mitochondrial bax, reduced the release of cytochrome c, and decreased the expression of caspase-3 and caspase-9 serially in cisplatin nephrotoxicity. Conclusion: We demonstrated that LA attenuates cisplatin-induced renal tubular damages by inhibition of mitochondrial bax translocation in vivo.

Original languageEnglish
JournalNephron - Experimental Nephrology
Volume113
Issue number4
DOIs
Publication statusPublished - 2009 Nov 1

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Thioctic Acid
Cisplatin
Wounds and Injuries
Caspase 9
Blood Urea Nitrogen
Apoptosis
Cytochromes c
Kidney
Caspase 3
Creatinine
Cell Death
Necrosis
Death Domain Receptors
DNA Nucleotidylexotransferase
Serum
Sulfhydryl Compounds
Sprague Dawley Rats
Microscopy
Antioxidants
Western Blotting

Keywords

  • Antioxidant
  • Apoptosis
  • Bax protein
  • Cisplatin
  • Mitochondria
  • Renal insufficiency
  • Thioctic acid

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Nephrology

Cite this

Alpha-lipoic acid attenuates cisplatin-induced tubulointerstitial injuries through inhibition of mitochondrial bax translocation in rats. / Lee, Young Mo; Bae, So Yon; Won, Nam Hee; Pyo, Heui Jung; Kwon, Young-Joo.

In: Nephron - Experimental Nephrology, Vol. 113, No. 4, 01.11.2009.

Research output: Contribution to journalArticle

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