Alteration of CD4+CD25+Foxp3+ T cell level in Kawasaki disease

Su Ye Sohn, Young Wooh Song, Yun Ku Yeo, Kyung Kim, Giyoung Jang, Chan Wook Woo, Jung Hwa Lee, Kwang Chul Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: Exaggerated pro-inflammatory reactions during the acute phase of Kawasaki disease (KD) suggest the role of immune dysregulation in the pathogenesis of KD. We investigated the profiles of T regulatory cells and their correlation with the clinical course of KD. Methods: Peripheral blood mononuclear cells were collected from 17 KD patients during acute febrile and subacute afebrile phases. T cells expressing CD4, CD25, and Foxp3 were analyzed using flow cytometry, and the results were correlated with the clinical course of KD. Results: The percentage of circulating CD4+CD25highFoxp3+ T cells among CD4+ T cells was significantly higher during the subacute afebrile phase than during the acute febrile phase (1.10%±1.22% vs. 0.55%±0.53%, P=0.049). Although levels of CD4+CD25lowFoxp3+ T cells and CD4+CD25. +Foxp3+ T cells were only slightly altered, the percentage of CD4+CD25-Foxp3+ T cells among CD4+ T cells was significantly lower during the subacute afebrile phase than during the acute febrile phase (2.96%±1.95% vs. 5.64%±5.69%, P=0.036). Consequently, the ratio of CD25highFoxp3+ T cells to CD25+Foxp3+ T cells was higher during the subacute afebrile phase than during the acute febrile phase (0.45%±0.57% vs. 0.13%±0.13%, P=0.038). Conclusion: Decreased CD4+CD25highFoxp3+ T cells and/or an imbalanced ratio of CD4+CD25highFoxp3+ T cells to CD4+CD25+ Foxp3+ T cells might play a role in KD development. Considering that all KD patients were treated with intravenous immunoglobulin (IVIG), recovery of CD4+CD25highFoxp3+ T cells during the subacute afebrile phase could be a mechanism of IVIG.

Original languageEnglish
Pages (from-to)157-162
Number of pages6
JournalKorean Journal of Pediatrics
Volume54
Issue number4
DOIs
Publication statusPublished - 2011 Jan 1

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Mucocutaneous Lymph Node Syndrome
T-Lymphocytes
Fever
Intravenous Immunoglobulins
Acute-Phase Reaction
Regulatory T-Lymphocytes
Blood Cells
Flow Cytometry

Keywords

  • Kawasaki disease
  • Regulatory T cell

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Pediatrics

Cite this

Alteration of CD4+CD25+Foxp3+ T cell level in Kawasaki disease. / Sohn, Su Ye; Song, Young Wooh; Yeo, Yun Ku; Kim, Kyung; Jang, Giyoung; Woo, Chan Wook; Lee, Jung Hwa; Lee, Kwang Chul.

In: Korean Journal of Pediatrics, Vol. 54, No. 4, 01.01.2011, p. 157-162.

Research output: Contribution to journalArticle

Sohn, Su Ye ; Song, Young Wooh ; Yeo, Yun Ku ; Kim, Kyung ; Jang, Giyoung ; Woo, Chan Wook ; Lee, Jung Hwa ; Lee, Kwang Chul. / Alteration of CD4+CD25+Foxp3+ T cell level in Kawasaki disease. In: Korean Journal of Pediatrics. 2011 ; Vol. 54, No. 4. pp. 157-162.
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abstract = "Purpose: Exaggerated pro-inflammatory reactions during the acute phase of Kawasaki disease (KD) suggest the role of immune dysregulation in the pathogenesis of KD. We investigated the profiles of T regulatory cells and their correlation with the clinical course of KD. Methods: Peripheral blood mononuclear cells were collected from 17 KD patients during acute febrile and subacute afebrile phases. T cells expressing CD4, CD25, and Foxp3 were analyzed using flow cytometry, and the results were correlated with the clinical course of KD. Results: The percentage of circulating CD4+CD25highFoxp3+ T cells among CD4+ T cells was significantly higher during the subacute afebrile phase than during the acute febrile phase (1.10{\%}±1.22{\%} vs. 0.55{\%}±0.53{\%}, P=0.049). Although levels of CD4+CD25lowFoxp3+ T cells and CD4+CD25. +Foxp3+ T cells were only slightly altered, the percentage of CD4+CD25-Foxp3+ T cells among CD4+ T cells was significantly lower during the subacute afebrile phase than during the acute febrile phase (2.96{\%}±1.95{\%} vs. 5.64{\%}±5.69{\%}, P=0.036). Consequently, the ratio of CD25highFoxp3+ T cells to CD25+Foxp3+ T cells was higher during the subacute afebrile phase than during the acute febrile phase (0.45{\%}±0.57{\%} vs. 0.13{\%}±0.13{\%}, P=0.038). Conclusion: Decreased CD4+CD25highFoxp3+ T cells and/or an imbalanced ratio of CD4+CD25highFoxp3+ T cells to CD4+CD25+ Foxp3+ T cells might play a role in KD development. Considering that all KD patients were treated with intravenous immunoglobulin (IVIG), recovery of CD4+CD25highFoxp3+ T cells during the subacute afebrile phase could be a mechanism of IVIG.",
keywords = "Kawasaki disease, Regulatory T cell",
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T1 - Alteration of CD4+CD25+Foxp3+ T cell level in Kawasaki disease

AU - Sohn, Su Ye

AU - Song, Young Wooh

AU - Yeo, Yun Ku

AU - Kim, Kyung

AU - Jang, Giyoung

AU - Woo, Chan Wook

AU - Lee, Jung Hwa

AU - Lee, Kwang Chul

PY - 2011/1/1

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N2 - Purpose: Exaggerated pro-inflammatory reactions during the acute phase of Kawasaki disease (KD) suggest the role of immune dysregulation in the pathogenesis of KD. We investigated the profiles of T regulatory cells and their correlation with the clinical course of KD. Methods: Peripheral blood mononuclear cells were collected from 17 KD patients during acute febrile and subacute afebrile phases. T cells expressing CD4, CD25, and Foxp3 were analyzed using flow cytometry, and the results were correlated with the clinical course of KD. Results: The percentage of circulating CD4+CD25highFoxp3+ T cells among CD4+ T cells was significantly higher during the subacute afebrile phase than during the acute febrile phase (1.10%±1.22% vs. 0.55%±0.53%, P=0.049). Although levels of CD4+CD25lowFoxp3+ T cells and CD4+CD25. +Foxp3+ T cells were only slightly altered, the percentage of CD4+CD25-Foxp3+ T cells among CD4+ T cells was significantly lower during the subacute afebrile phase than during the acute febrile phase (2.96%±1.95% vs. 5.64%±5.69%, P=0.036). Consequently, the ratio of CD25highFoxp3+ T cells to CD25+Foxp3+ T cells was higher during the subacute afebrile phase than during the acute febrile phase (0.45%±0.57% vs. 0.13%±0.13%, P=0.038). Conclusion: Decreased CD4+CD25highFoxp3+ T cells and/or an imbalanced ratio of CD4+CD25highFoxp3+ T cells to CD4+CD25+ Foxp3+ T cells might play a role in KD development. Considering that all KD patients were treated with intravenous immunoglobulin (IVIG), recovery of CD4+CD25highFoxp3+ T cells during the subacute afebrile phase could be a mechanism of IVIG.

AB - Purpose: Exaggerated pro-inflammatory reactions during the acute phase of Kawasaki disease (KD) suggest the role of immune dysregulation in the pathogenesis of KD. We investigated the profiles of T regulatory cells and their correlation with the clinical course of KD. Methods: Peripheral blood mononuclear cells were collected from 17 KD patients during acute febrile and subacute afebrile phases. T cells expressing CD4, CD25, and Foxp3 were analyzed using flow cytometry, and the results were correlated with the clinical course of KD. Results: The percentage of circulating CD4+CD25highFoxp3+ T cells among CD4+ T cells was significantly higher during the subacute afebrile phase than during the acute febrile phase (1.10%±1.22% vs. 0.55%±0.53%, P=0.049). Although levels of CD4+CD25lowFoxp3+ T cells and CD4+CD25. +Foxp3+ T cells were only slightly altered, the percentage of CD4+CD25-Foxp3+ T cells among CD4+ T cells was significantly lower during the subacute afebrile phase than during the acute febrile phase (2.96%±1.95% vs. 5.64%±5.69%, P=0.036). Consequently, the ratio of CD25highFoxp3+ T cells to CD25+Foxp3+ T cells was higher during the subacute afebrile phase than during the acute febrile phase (0.45%±0.57% vs. 0.13%±0.13%, P=0.038). Conclusion: Decreased CD4+CD25highFoxp3+ T cells and/or an imbalanced ratio of CD4+CD25highFoxp3+ T cells to CD4+CD25+ Foxp3+ T cells might play a role in KD development. Considering that all KD patients were treated with intravenous immunoglobulin (IVIG), recovery of CD4+CD25highFoxp3+ T cells during the subacute afebrile phase could be a mechanism of IVIG.

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