Altered branching patterns of Purkinje cells in mouse model for cortical development disorder

Jinkyung Kim, Namseop Kwon, Soeun Chang, Kyong Tai Kim, Dongmyeong Lee, Seunghwan Kim, So Jeong Yun, Daehee Hwang, Jee Woong Kim, Yeukuang Hwu, Giorgio Margaritondo, Jung Ho Je, I. J.Im Joo Rhyu

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11 Citations (Scopus)

Abstract

Disrupted cortical cytoarchitecture in cerebellum is a typical pathology in reeler. Particularly interesting are structural problems at the cellular level: dendritic morphology has important functional implication in signal processing. Here we describe a combinatorial imaging method of synchrotron X-ray microtomography with Golgi staining, which can deliver 3-dimensional(3-D) micro-architectures of Purkinje cell(PC) dendrites, and give access to quantitative information in 3-D geometry. In reeler, we visualized in 3-D geometry the shape alterations of planar PC dendrites (i.e., abnormal 3-D arborization). Despite these alterations, the 3-D quantitative analysis of the branching patterns showed no significant changes of the 77 ± 8° branch angle, whereas the branch segment length strongly increased with large fluctuations, comparing to control. The 3-D fractal dimension of the PCs decreased from 1.723 to 1.254, indicating a significant reduction of dendritic complexity. This study provides insights into etiologies and further potential treatment options for lissencephaly and various neurodevelopmental disorders.

Original languageEnglish
Article number122
JournalScientific reports
Volume1
DOIs
Publication statusPublished - 2011

ASJC Scopus subject areas

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    Kim, J., Kwon, N., Chang, S., Kim, K. T., Lee, D., Kim, S., Yun, S. J., Hwang, D., Kim, J. W., Hwu, Y., Margaritondo, G., Je, J. H., & Rhyu, I. J. I. J. (2011). Altered branching patterns of Purkinje cells in mouse model for cortical development disorder. Scientific reports, 1, [122]. https://doi.org/10.1038/srep00122