Altered expression of synaptotagmin 13 mRNA in adult mouse brain after contextual fear conditioning

Seungrie Han, Soontaek Hong, Dongmin Lee, Myeong Hoe Lee, June Seek Choi, Min Jung Koh, Woong Sun, Hyun Kim, Hyun Woo Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Contextual fear memory processing requires coordinated changes in neuronal activity and molecular networks within brain. A large number of fear memory-related genes, however, still remain to be identified. Synaptotagmin 13 (Syt13), an atypical member of synaptotagmin family, is highly expressed in brain, but its functional roles within brain have not yet been clarified. Here, we report that the expression of Syt13 mRNA in adult mouse brain was altered following contextual fear conditioning. C57BL/6 mice were exposed to a novel context and stimulated by strong electrical footshock according to a contextual fear conditioning protocol. After 24. h, the mice were re-exposed to the context without electrical footshock for the retrieval of contextual fear memory. To investigate the relationship between Syt13 and contextual fear memory, we carried out in situ hybridization and analyzed gene expression patterns for Syt13 at four groups representing temporal changes in brain activity during contextual fear memory formation. Contextual fear conditioning test induced significant changes in mRNA levels for Syt13 within various brain regions, including lateral amygdala, somatosensory cortex, piriform cortex, habenula, thalamus, and hypothalamus, during both acquisition and retrieval sessions. Our data suggest that Syt13 may be involved in the process of contextual fear memory.

Original languageEnglish
Pages (from-to)880-885
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume425
Issue number4
DOIs
Publication statusPublished - 2012 Sep 7

Fingerprint

Synaptotagmins
Fear
Brain
Messenger RNA
Data storage equipment
Habenula
Conditioning (Psychology)
Gene expression
Somatosensory Cortex
Amygdala
Thalamus
Inbred C57BL Mouse
Genes
Hypothalamus
In Situ Hybridization
Processing

Keywords

  • Contextual fear conditioning
  • In situ hybridization
  • Syt13

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Altered expression of synaptotagmin 13 mRNA in adult mouse brain after contextual fear conditioning. / Han, Seungrie; Hong, Soontaek; Lee, Dongmin; Lee, Myeong Hoe; Choi, June Seek; Koh, Min Jung; Sun, Woong; Kim, Hyun; Lee, Hyun Woo.

In: Biochemical and Biophysical Research Communications, Vol. 425, No. 4, 07.09.2012, p. 880-885.

Research output: Contribution to journalArticle

Han, Seungrie ; Hong, Soontaek ; Lee, Dongmin ; Lee, Myeong Hoe ; Choi, June Seek ; Koh, Min Jung ; Sun, Woong ; Kim, Hyun ; Lee, Hyun Woo. / Altered expression of synaptotagmin 13 mRNA in adult mouse brain after contextual fear conditioning. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 425, No. 4. pp. 880-885.
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