TY - JOUR
T1 - Altered heart and kidney phospholipid fatty acid composition are associated with cardiac hypertrophy in hypertensive rats
AU - Kim, Oh Yoen
AU - Jung, Young Sang
AU - Cho, Yoonsu
AU - Chung, Ji Hyung
AU - Hwang, Geum Sook
AU - Shin, Min Jeong
N1 - Funding Information:
This research was supported by the Basic Science Research Program through the National research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2012-0002119 ), the Creative Allied Project (CAP) grant funded by the Korea Research Council of Fundamental Science and Technology (KRCF) and Korea Basic Science Institute ( E33300 ).
PY - 2013/8
Y1 - 2013/8
N2 - Objective: We examined the association of cardiac hypertrophy or fibrosis with the phospholipid fatty acid (FA) composition of heart and kidney in hypertensive rats. Design and methods: Eight-week-old spontaneously hypertensive rats (SHRs) (n. =. 8) and Wistar Kyoto rats (WKYs, n. =. 8) as a normotensive control, were fed ad libitum for 6. weeks with regular AIN-76 diet. Phospholipid FA compositions in the left ventricle and kidney were measured and histological analyses were performed. Results: Compared with WKYs, SHRs had lower proportions of γ-linolenic acid, α-linolenic acid, eicosadienoic acid, eicosatrienoic acid, dihomo-γ-linoleic acid, docosadienoic acid and nervonic acid in heart, and stearic acid (SA), γ-linolenic acid, and eicosapentaenoic acid (EPA) in kidney. After adjusting for food intake, SHRs still maintained higher proportions of SA, and total saturated FAs in the heart and a lower proportion of eicosapentaenoic acid in the kidney. Additionally, compared with WKYs, SHRs showed larger cardiomyocyte diameters in the left ventricles, indicating cardiac hypertrophy and interstitial fibrosis. Cardiomyocyte diameters also positively correlated with cardiac SA (r. =. 0.550, p. <. 0.05) and negatively with kidney EPA (r. =. -. 0.575, p. <. 0.05). Conclusion: Tissue FA compositions were associated with cardiac hypertrophy in a hypertensive setting, implicating the pathogenic role of tissue FAs in hypertension and related complications.
AB - Objective: We examined the association of cardiac hypertrophy or fibrosis with the phospholipid fatty acid (FA) composition of heart and kidney in hypertensive rats. Design and methods: Eight-week-old spontaneously hypertensive rats (SHRs) (n. =. 8) and Wistar Kyoto rats (WKYs, n. =. 8) as a normotensive control, were fed ad libitum for 6. weeks with regular AIN-76 diet. Phospholipid FA compositions in the left ventricle and kidney were measured and histological analyses were performed. Results: Compared with WKYs, SHRs had lower proportions of γ-linolenic acid, α-linolenic acid, eicosadienoic acid, eicosatrienoic acid, dihomo-γ-linoleic acid, docosadienoic acid and nervonic acid in heart, and stearic acid (SA), γ-linolenic acid, and eicosapentaenoic acid (EPA) in kidney. After adjusting for food intake, SHRs still maintained higher proportions of SA, and total saturated FAs in the heart and a lower proportion of eicosapentaenoic acid in the kidney. Additionally, compared with WKYs, SHRs showed larger cardiomyocyte diameters in the left ventricles, indicating cardiac hypertrophy and interstitial fibrosis. Cardiomyocyte diameters also positively correlated with cardiac SA (r. =. 0.550, p. <. 0.05) and negatively with kidney EPA (r. =. -. 0.575, p. <. 0.05). Conclusion: Tissue FA compositions were associated with cardiac hypertrophy in a hypertensive setting, implicating the pathogenic role of tissue FAs in hypertension and related complications.
KW - Cardiac hypertrophy
KW - Fatty acid
KW - Hypertension
KW - Kidney
KW - Left ventricle
UR - http://www.scopus.com/inward/record.url?scp=84880630633&partnerID=8YFLogxK
U2 - 10.1016/j.clinbiochem.2013.04.008
DO - 10.1016/j.clinbiochem.2013.04.008
M3 - Article
C2 - 23608354
AN - SCOPUS:84880630633
SN - 0009-9120
VL - 46
SP - 1111
EP - 1117
JO - Clinical Biochemistry
JF - Clinical Biochemistry
IS - 12
ER -