Altered monocyte-derived dendritic cell function in patients on hemodialysis: A culprit for underlying impaired immune responses

Hye Min Choi, Young Seok Woo, Myung Gyu Kim, Sang Kyung Jo, Won Yong Cho, Hyoung Kyu Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background Patients with end-stage renal disease (ESRD) are known to have impaired immune function. Dendritic cells (DCs) are the major antigen-presenting cells that initiate primary immune responses, linking innate and adaptive immunity. Although suboptimal immune responses to vaccination, as frequently observed in ESRD patients, might suggest the presence of impaired DC function, the precise nature of altered DC function is not fully understood. Methods In the current study, we compared the maturation status, viability, and function of monocyte-derived DCs (moDCs) of patients on hemodialysis (HD) with healthy controls. Results Surface expression of major histocompatibility complex class II, CD83, and CD86, and chemokine receptor CCR7 in moDCs was not different between HD patients and healthy controls. No significant difference was detected in the viability of moDCs determined by expression of annexin V and propidium iodide between two groups. However, moDCs from HD patients produced significantly higher amounts of IL-6 when stimulated by cytokine cocktails compared to healthy controls. In addition, mature moDCs from HD patients showed significantly enhanced allogeneic T-cell proliferation compared to healthy controls. Conclusions Our data demonstrate aberrant DC function in HD patients and suggest that this might contribute to impaired immune responses.

Original languageEnglish
Pages (from-to)546-553
Number of pages8
JournalClinical and Experimental Nephrology
Volume15
Issue number4
DOIs
Publication statusPublished - 2011 Aug

Keywords

  • End-stage renal disease
  • Function of dendritic cells
  • Immune responses

ASJC Scopus subject areas

  • Physiology
  • Nephrology
  • Physiology (medical)

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