Alternative splicing and expression analysis of High expression of osmotically responsive genes1 (HOS1) in Arabidopsis

Jeong Hwan Lee, Soo Hyun Kim, Jae Joon Kim, Ji Hoon Ahn

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9 Citations (Scopus)

Abstract

High expression of osmotically responsive genes1 (HOS1), a key regulator of low temperature response and flowering time, encodes an E3 ubiquitin ligase in Arabidopsis. Here, we report characterization of a newly identified splice variant (HOS1-L) of HOS1. Comparative analyses revealed that HOS1-L has a longer 5' nucleotide sequence than that of the previously identified HOS1 (HOS1-S) and that itsprotein sequence was more conserved than that of HOS1-S in plants. HOS1-L transcripts were spatio-temporally more abundant than those of HOS1-S. The recovery rate of HOS1-S expression was faster than that of HOS1-L after cold treatment. Diurnal oscillation patterns of HOS1-L revealed that HOS1-L expression was affected by photoperiod. An in vitro pull-down assay revealed that the HOS1-L protein interacted with the ICE1 protein. HOS1-L overexpression caused delayed flowering in wild-type plants. Collectively, these results suggest regulation of HOS1 expression at the post-transcriptional level.

Original languageEnglish
Pages (from-to)515-520
Number of pages6
JournalBMB Reports
Volume45
Issue number9
DOIs
Publication statusPublished - 2012 Dec 27

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Keywords

  • AAB87130
  • Alternative splicing
  • BT005517
  • HOS1
  • Posttranscriptional regulation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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