Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs

Jaemoon Yang; Eun Sook Lee; Min Young Noh; Seong Ho Koh; Eun Kyung Lim; A. Rum Yoo; Kwangyeol Lee; Jin Suck Suh; Seung Hyun Kim; Seungjoo Haam; Yong Min Huh

doi:10.1016/j.biomaterials.2011.04.007

Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs

Jaemoon Yang, Eun Sook Lee, Min Young Noh, Seong Ho Koh, Eun Kyung Lim, A. Rum Yoo, Kwangyeol Lee, Jin Suck Suh, Seung Hyun Kim, Seungjoo Haam, Yong Min Huh

Department of Chemistry

Research output: Contribution to journal › Article

18 Citations (Scopus)

Abstract

The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar aprotic solvent soluble MnFe ₂O ₄ nanoparticles were enveloped using cationic polymers (branched polyethyleneimine, PEI) by the solvent shifting method for a gene loading. Using our magnetic nanovector system (PEI-coated MnFe ₂O ₄ nanoparticles), thus, we synchronized stem cell migration and its gene expression in a rat stroke model.

Original language	English
Pages (from-to)	6174-6182
Number of pages	9
Journal	Biomaterials
Volume	32
Issue number	26
DOIs	https://doi.org/10.1016/j.biomaterials.2011.04.007
Publication status	Published - 2011 Sep 1

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Keywords

Gene delivery
Magnetic
Nanovectors
Solvent shifting
Stem cell

ASJC Scopus subject areas

Biomaterials
Bioengineering
Ceramics and Composites
Mechanics of Materials
Biophysics

Cite this

Yang, J., Lee, E. S., Noh, M. Y., Koh, S. H., Lim, E. K., Yoo, A. R., Lee, K., Suh, J. S., Kim, S. H., Haam, S., & Huh, Y. M. (2011). Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs. Biomaterials, 32(26), 6174-6182. https://doi.org/10.1016/j.biomaterials.2011.04.007

Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs. / Yang, Jaemoon; Lee, Eun Sook; Noh, Min Young; Koh, Seong Ho; Lim, Eun Kyung; Yoo, A. Rum; Lee, Kwangyeol; Suh, Jin Suck; Kim, Seung Hyun; Haam, Seungjoo; Huh, Yong Min.

In: Biomaterials, Vol. 32, No. 26, 01.09.2011, p. 6174-6182.

Research output: Contribution to journal › Article

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10.1016/j.biomaterials.2011.04.007