Abstract
The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar aprotic solvent soluble MnFe 2O 4 nanoparticles were enveloped using cationic polymers (branched polyethyleneimine, PEI) by the solvent shifting method for a gene loading. Using our magnetic nanovector system (PEI-coated MnFe 2O 4 nanoparticles), thus, we synchronized stem cell migration and its gene expression in a rat stroke model.
Original language | English |
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Pages (from-to) | 6174-6182 |
Number of pages | 9 |
Journal | Biomaterials |
Volume | 32 |
Issue number | 26 |
DOIs | |
Publication status | Published - 2011 Sep |
Keywords
- Gene delivery
- Magnetic
- Nanovectors
- Solvent shifting
- Stem cell
ASJC Scopus subject areas
- Biophysics
- Bioengineering
- Ceramics and Composites
- Biomaterials
- Mechanics of Materials