Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs

Jaemoon Yang; Eun Sook Lee; Min Young Noh; Seong Ho Koh; Eun Kyung Lim; A. Rum Yoo; Kwangyeol Lee; Jin Suck Suh; Seung Hyun Kim; Seungjoo Haam; Yong Min Huh

doi:10.1016/j.biomaterials.2011.04.007

Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs

Jaemoon Yang, Eun Sook Lee, Min Young Noh, Seong Ho Koh, Eun Kyung Lim, A. Rum Yoo, Kwangyeol Lee, Jin Suck Suh, Seung Hyun Kim, Seungjoo Haam, Yong Min Huh

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar aprotic solvent soluble MnFe ₂O ₄ nanoparticles were enveloped using cationic polymers (branched polyethyleneimine, PEI) by the solvent shifting method for a gene loading. Using our magnetic nanovector system (PEI-coated MnFe ₂O ₄ nanoparticles), thus, we synchronized stem cell migration and its gene expression in a rat stroke model.

Original language	English
Pages (from-to)	6174-6182
Number of pages	9
Journal	Biomaterials
Volume	32
Issue number	26
DOIs	https://doi.org/10.1016/j.biomaterials.2011.04.007
Publication status	Published - 2011 Sep

Keywords

Gene delivery
Magnetic
Nanovectors
Solvent shifting
Stem cell

ASJC Scopus subject areas

Biophysics
Bioengineering
Ceramics and Composites
Biomaterials
Mechanics of Materials

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10.1016/j.biomaterials.2011.04.007

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Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs. / Yang, Jaemoon; Lee, Eun Sook; Noh, Min Young; Koh, Seong Ho; Lim, Eun Kyung; Yoo, A. Rum; Lee, Kwangyeol; Suh, Jin Suck; Kim, Seung Hyun; Haam, Seungjoo; Huh, Yong Min.

In: Biomaterials, Vol. 32, No. 26, 09.2011, p. 6174-6182.

Research output: Contribution to journal › Article › peer-review

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title = "Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs",

abstract = "The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar aprotic solvent soluble MnFe 2O 4 nanoparticles were enveloped using cationic polymers (branched polyethyleneimine, PEI) by the solvent shifting method for a gene loading. Using our magnetic nanovector system (PEI-coated MnFe 2O 4 nanoparticles), thus, we synchronized stem cell migration and its gene expression in a rat stroke model.",

keywords = "Gene delivery, Magnetic, Nanovectors, Solvent shifting, Stem cell",

author = "Jaemoon Yang and Lee, {Eun Sook} and Noh, {Min Young} and Koh, {Seong Ho} and Lim, {Eun Kyung} and Yoo, {A. Rum} and Kwangyeol Lee and Suh, {Jin Suck} and Kim, {Seung Hyun} and Seungjoo Haam and Huh, {Yong Min}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST) ( 2010-0020587 ) and a grantof the Korea Healthcare technology R&D Project, Ministry for Health & Welfare Affairs, Republic of Korea ( A085136 ).",

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AU - Yoo, A. Rum

AU - Lee, Kwangyeol

AU - Suh, Jin Suck

AU - Kim, Seung Hyun

AU - Haam, Seungjoo

AU - Huh, Yong Min

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AB - The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar aprotic solvent soluble MnFe 2O 4 nanoparticles were enveloped using cationic polymers (branched polyethyleneimine, PEI) by the solvent shifting method for a gene loading. Using our magnetic nanovector system (PEI-coated MnFe 2O 4 nanoparticles), thus, we synchronized stem cell migration and its gene expression in a rat stroke model.

KW - Gene delivery

KW - Magnetic

KW - Nanovectors

KW - Solvent shifting

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Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs

Abstract

Keywords

ASJC Scopus subject areas

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