Abstract
The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar aprotic solvent soluble MnFe 2O 4 nanoparticles were enveloped using cationic polymers (branched polyethyleneimine, PEI) by the solvent shifting method for a gene loading. Using our magnetic nanovector system (PEI-coated MnFe 2O 4 nanoparticles), thus, we synchronized stem cell migration and its gene expression in a rat stroke model.
| Original language | English |
|---|---|
| Pages (from-to) | 6174-6182 |
| Number of pages | 9 |
| Journal | Biomaterials |
| Volume | 32 |
| Issue number | 26 |
| DOIs | |
| Publication status | Published - 2011 Sep 1 |
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Keywords
- Gene delivery
- Magnetic
- Nanovectors
- Solvent shifting
- Stem cell
ASJC Scopus subject areas
- Biomaterials
- Bioengineering
- Ceramics and Composites
- Mechanics of Materials
- Biophysics
Cite this
Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs. / Yang, Jaemoon; Lee, Eun Sook; Noh, Min Young; Koh, Seong Ho; Lim, Eun Kyung; Yoo, A. Rum; Lee, Kwangyeol; Suh, Jin Suck; Kim, Seung Hyun; Haam, Seungjoo; Huh, Yong Min.
In: Biomaterials, Vol. 32, No. 26, 01.09.2011, p. 6174-6182.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs
AU - Yang, Jaemoon
AU - Lee, Eun Sook
AU - Noh, Min Young
AU - Koh, Seong Ho
AU - Lim, Eun Kyung
AU - Yoo, A. Rum
AU - Lee, Kwangyeol
AU - Suh, Jin Suck
AU - Kim, Seung Hyun
AU - Haam, Seungjoo
AU - Huh, Yong Min
PY - 2011/9/1
Y1 - 2011/9/1
N2 - The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar aprotic solvent soluble MnFe 2O 4 nanoparticles were enveloped using cationic polymers (branched polyethyleneimine, PEI) by the solvent shifting method for a gene loading. Using our magnetic nanovector system (PEI-coated MnFe 2O 4 nanoparticles), thus, we synchronized stem cell migration and its gene expression in a rat stroke model.
AB - The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar aprotic solvent soluble MnFe 2O 4 nanoparticles were enveloped using cationic polymers (branched polyethyleneimine, PEI) by the solvent shifting method for a gene loading. Using our magnetic nanovector system (PEI-coated MnFe 2O 4 nanoparticles), thus, we synchronized stem cell migration and its gene expression in a rat stroke model.
KW - Gene delivery
KW - Magnetic
KW - Nanovectors
KW - Solvent shifting
KW - Stem cell
UR - http://www.scopus.com/inward/record.url?scp=79959914365&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79959914365&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2011.04.007
DO - 10.1016/j.biomaterials.2011.04.007
M3 - Article
C2 - 21696819
AN - SCOPUS:79959914365
VL - 32
SP - 6174
EP - 6182
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 26
ER -