Ameliorating effect of Gardenia jasminoides extract on amyloid beta peptide-induced neuronal cell deficit

Soo Jung Choi, Mi Jeong Kim, Ho Jin Heo, Bumshik Hong, Hong Yon Cho, Young Jun Kim, Hye Kyung Kim, Seung Taik Lim, Woo Jin Jun, Eun Ki Kim, Dong Hoon Shin

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The brains of Alzheimer's disease (AD) patients are characterized by large deposits of amyloid beta peptide (AP). Aβ is known to increase free radical production in nerve cells, leading to cell death that is characterized by lipid peroxidation, free radical formation, protein oxidation, and DNA/RNA oxidation. In this study, we selected an extract of Gardenia jasminoides by screening, and investigated its ameliorating effects on Aβ-induced oxidative stress using PC12 cells. The effects of the extract were evaluated using the 2′,7′-dichlorofluorescein diacetate (DCF-DA) assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. To find the active component, the ethanol extract was partitioned with hexane, chloroform, and ethyl acetate, respectively, and the active component was purified by silica-gel column chromatography and HPLC. The results suggested that Gardenia jasminoides extract can reduce the cytotoxicity of Aβ in PC 12 cells, possibly by reducing oxidative stress.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalMolecules and cells
Volume24
Issue number1
Publication statusPublished - 2007 Aug 31

Keywords

  • Alzheimer's disease
  • Amyloid beta
  • Gardenia jasminoides
  • Oxidative stress
  • PC12 cells

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Choi, S. J., Kim, M. J., Heo, H. J., Hong, B., Cho, H. Y., Kim, Y. J., Kim, H. K., Lim, S. T., Jun, W. J., Kim, E. K., & Shin, D. H. (2007). Ameliorating effect of Gardenia jasminoides extract on amyloid beta peptide-induced neuronal cell deficit. Molecules and cells, 24(1), 113-118.