Amelioration of cerebral infarction and improvement of neurological deficit by a Korean herbal medicine, modified Bo-Yang-Hwan-O-Tang

Yookeum Choi, Seul Ki Kim, In Young Choi, Chung Ju, Kung Woo Nam, Sunyoung Hwang, Byung Woo Kim, Min Ji Yoon, Moo Ho Won, Yong Ki Park, Won-Ki Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objectives Modified Bo-Yang-Hwan-O-Tang (mBHT) is an improved herbal formula of BHT, which has been widely used to treat ischaemic stroke in East Asia, by the addition of five herbs having anti-ischaemic properties. In this study, we investigated whether mBHT would reduce cerebral ischaemic injury in rats. Methods Sprague-Dawley rats were subjected to a 90-min middle cerebral artery occlusion (MCAO) and subsequent 22-h reperfusion. mBHT was administered either intraperitoneally twice 15 min before and 15 min after, or orally once 30 min or 120 min after the onset of MCAO (50 or 200 mg/kg each). Key findings Intraperitoneal administration of mBHT markedly reduced the cerebral infarct size and neurological deficit caused by MCAO/reperfusion. mBHT treatment also significantly improved long-term survival rate after cerebral ischaemic injury. Oral administration of mBHT 30 min after ischaemia also markedly reduced the infarct size after cerebral ischaemia. The anti-ischaemic effect of mBHT was significantly, but not fully, reduced when mBHT-induced hypothermia was abolished. In cultured cortical neurons, we further found that mBHT decreased oxygen-glucose deprivation/re-oxygenation-evoked neuronal injury by inhibiting production of reactive oxygen species, decrease in mitochondrial transmembrane potential, and activation of caspase-3. However, mBHT did not inhibit N-Methyl-D-aspartate (NMDA) receptor-mediated excitotoxicity. Conclusions Taken together, our data suggest that mBHT has multiple anti-ischaemic properties and would be a good therapeutic herbal prescription for the treatment of cerebral ischaemic stroke.

Original languageEnglish
Pages (from-to)695-706
Number of pages12
JournalJournal of Pharmacy and Pharmacology
Volume63
Issue number5
DOIs
Publication statusPublished - 2011 May 1

    Fingerprint

Keywords

  • hypothermia
  • ischaemic injury
  • mBHT
  • OGD
  • ROS

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this