Amelioration of cerebral infarction and improvement of neurological deficit by a Korean herbal medicine, modified Bo-Yang-Hwan-O-Tang

Yookeum Choi, Seul Ki Kim, In Young Choi, Chung Ju, Kung Woo Nam, Sunyoung Hwang, Byung Woo Kim, Min Ji Yoon, Moo Ho Won, Yong Ki Park, Won Ki Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objectives Modified Bo-Yang-Hwan-O-Tang (mBHT) is an improved herbal formula of BHT, which has been widely used to treat ischaemic stroke in East Asia, by the addition of five herbs having anti-ischaemic properties. In this study, we investigated whether mBHT would reduce cerebral ischaemic injury in rats. Methods Sprague-Dawley rats were subjected to a 90-min middle cerebral artery occlusion (MCAO) and subsequent 22-h reperfusion. mBHT was administered either intraperitoneally twice 15 min before and 15 min after, or orally once 30 min or 120 min after the onset of MCAO (50 or 200 mg/kg each). Key findings Intraperitoneal administration of mBHT markedly reduced the cerebral infarct size and neurological deficit caused by MCAO/reperfusion. mBHT treatment also significantly improved long-term survival rate after cerebral ischaemic injury. Oral administration of mBHT 30 min after ischaemia also markedly reduced the infarct size after cerebral ischaemia. The anti-ischaemic effect of mBHT was significantly, but not fully, reduced when mBHT-induced hypothermia was abolished. In cultured cortical neurons, we further found that mBHT decreased oxygen-glucose deprivation/re-oxygenation-evoked neuronal injury by inhibiting production of reactive oxygen species, decrease in mitochondrial transmembrane potential, and activation of caspase-3. However, mBHT did not inhibit N-Methyl-D-aspartate (NMDA) receptor-mediated excitotoxicity. Conclusions Taken together, our data suggest that mBHT has multiple anti-ischaemic properties and would be a good therapeutic herbal prescription for the treatment of cerebral ischaemic stroke.

Original languageEnglish
Pages (from-to)695-706
Number of pages12
JournalJournal of Pharmacy and Pharmacology
Volume63
Issue number5
DOIs
Publication statusPublished - 2011 May

Keywords

  • OGD
  • ROS
  • hypothermia
  • ischaemic injury
  • mBHT

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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