Amino acid sequence analysis of bitter peptides from a soybean proglycinin subunit synthesized in Escherichia coli

Mi Ryung Kim, Sang-Yun Choi, Chan Shick Kim, Chan Wha Kim, Shigeru Utsumi, Cherl Ho Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The cDNA encoding A1aB1b), proglycinin was expressed in E. coli, for the efficient isolation of a single peptide responsible for the bitterness. The 55-kD proglycinin was highly purified, hydrolyzed, and further purified through a series of chromatographic steps to yield fractions with the major bitter peptides. The most bitter-tasting fractions contained peptides with average molecular weights lower than 1,700 Da. An analysis of the amino acid sequences indicated that many small bitter peptides (< 1,000 Da) are composed of uncharged polar amino acids as well as hydrophobic amino acids, with a charged residue often being present at either end. This suggests the involvement of a certain structural requirement in taste perception.

Original languageEnglish
Pages (from-to)2069-2074
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume63
Issue number12
Publication statusPublished - 1999 Dec 1

Fingerprint

Protein Sequence Analysis
Soybeans
Escherichia coli
Peptides
Amino acids
amino acid sequences
sequence analysis
peptides
soybeans
Amino Acids
Taste Perception
amino acids
bitterness
Complementary DNA
Molecular Weight
Molecular weight
molecular weight

Keywords

  • Bitter peptide
  • Proglycinin
  • Soybean protein hydrolysate

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Amino acid sequence analysis of bitter peptides from a soybean proglycinin subunit synthesized in Escherichia coli. / Kim, Mi Ryung; Choi, Sang-Yun; Kim, Chan Shick; Kim, Chan Wha; Utsumi, Shigeru; Lee, Cherl Ho.

In: Bioscience, Biotechnology and Biochemistry, Vol. 63, No. 12, 01.12.1999, p. 2069-2074.

Research output: Contribution to journalArticle

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