Aminoacyl tRNA synthetase-interacting multifunctional protein 1 acts as a novel B cell-activating factor in vitro and in vivo

Myun Soo Kim, Tae Sung Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Endogenous B cell-activating factors play pivotal roles in defense mechanisms by regulating B cell responses. We previously reported that aminoacyl tRNA synthetase-interacting multifunctional protein 1 (AIMP1) functions as a novel proinflammatory cytokine that activates macrophages and dendritic cells. However, roles of AIMP1 in B cell responses have not been studied. In this study, we investigated the effects of AIMP1 on B cell responses and their underlying mechanisms. AIMP1 induced the expression of surface activation markers on murine B cells and the proliferation of B cells. Additionally, AIMP1 increased the expression of activation-induced deaminase and class switch recombination in B cells. AIMP1 also had synergistic effects on B cell activation when combined with CD40 stimulus. Intracellular signaling experiments showed that AIMP1 activated B cells through a protein kinase C/NF-κB signaling pathway. Importantly, i.v. injection of AIMP1 into mice increased the expression of CD69 on splenic B cells and significantly enhanced Ag-specific Ab production. Taken together, our results show that AIMP1 acts as a novel B cell-activating factor. AIMP1-mediated B cell activation and the involvement of AIMP1 in diseases will provide additional information for therapeutic strategies.

Original languageEnglish
Pages (from-to)4729-4736
Number of pages8
JournalJournal of Immunology
Volume194
Issue number10
DOIs
Publication statusPublished - 2015 May 15

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B-Cell Activating Factor
Amino Acyl-tRNA Synthetases
B-Lymphocytes
Proteins
In Vitro Techniques
Dendritic Cells
Protein Kinase C
Genetic Recombination
Macrophages

ASJC Scopus subject areas

  • Immunology

Cite this

Aminoacyl tRNA synthetase-interacting multifunctional protein 1 acts as a novel B cell-activating factor in vitro and in vivo. / Kim, Myun Soo; Kim, Tae Sung.

In: Journal of Immunology, Vol. 194, No. 10, 15.05.2015, p. 4729-4736.

Research output: Contribution to journalArticle

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