AMP-activated protein kinase activity is critical for hypoxia-inducible factor-1 transcriptional activity and its target gene expression under hypoxic conditions in DU145 cells

Minyoung Lee, Jin Taek Hwang, Hye Jeong Lee, Seung N. Jung, Insug Kang, Sung-Gil Chi, Sung S. Kim, Joohun Ha

Research output: Contribution to journalArticle

207 Citations (Scopus)

Abstract

AMP-activated protein kinase (AMPK) functions as an energy sensor to provide metabolic adaptations under the ATP-deprived conditions such as hypoxia. In the present study, we considered a role of AMPK in the adaptive response to hypoxia by examining whether AMPK is involved in the regulation of hypoxia-inducible factor-1 (HIF-1), a heterodimeric transcription factor that is critical for hypoxic induction of physiologically important genes. We demonstrate that hypoxia or CoCl2 rapidly activated AMPK in DU145 human prostate cancer cells, and its activation preceded the induction of HIF-1α expression. Under these conditions, blockade of AMPK activity by a pharmacological or molecular approach significantly attenuated hypoxia-induced responses such as HIF-1 target gene expression, secretion of vascular endothelial growth factor, glucose uptake, and HIF-1-dependent reporter gene expression, indicating that AMPK is critical for the HIF-1 transcriptional activity and its target gene expression. Its functional requirement for HIF-1 activity was also demonstrated in several different cancer cell lines, but AMPK activation alone was not sufficient to stimulate the HIF-1 transcriptional activity. We further present data showing that AMPK transmits a positive signal for HIF-1 activity via a signaling pathway that is independent of phosphatidylinositol 3-kinase/AKT and several mitogen-activated protein kinases. Taken together, our results suggest that AMPK is a novel and critical component of HIF-1 regulation, implying its new roles in oxygen-regulated cellular phenomena.

Original languageEnglish
Pages (from-to)39653-39661
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number41
DOIs
Publication statusPublished - 2003 Oct 10
Externally publishedYes

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Hypoxia-Inducible Factor 1
AMP-Activated Protein Kinases
Gene expression
Gene Expression
Chemical activation
Cells
Phosphatidylinositol 3-Kinase
Mitogen-Activated Protein Kinases
Reporter Genes
Vascular Endothelial Growth Factor A
Prostatic Neoplasms
Transcription Factors
Genes
Adenosine Triphosphate
Pharmacology
Oxygen
Glucose
Cell Line

ASJC Scopus subject areas

  • Biochemistry

Cite this

AMP-activated protein kinase activity is critical for hypoxia-inducible factor-1 transcriptional activity and its target gene expression under hypoxic conditions in DU145 cells. / Lee, Minyoung; Hwang, Jin Taek; Lee, Hye Jeong; Jung, Seung N.; Kang, Insug; Chi, Sung-Gil; Kim, Sung S.; Ha, Joohun.

In: Journal of Biological Chemistry, Vol. 278, No. 41, 10.10.2003, p. 39653-39661.

Research output: Contribution to journalArticle

Lee, Minyoung ; Hwang, Jin Taek ; Lee, Hye Jeong ; Jung, Seung N. ; Kang, Insug ; Chi, Sung-Gil ; Kim, Sung S. ; Ha, Joohun. / AMP-activated protein kinase activity is critical for hypoxia-inducible factor-1 transcriptional activity and its target gene expression under hypoxic conditions in DU145 cells. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 41. pp. 39653-39661.
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