AMP-activated protein kinase and glycogen synthase kinase 3β modulate the severity of sepsis-induced lung injury

Zhongyu Liu, Nathaniel Bone, Shaoning Jiang, Dae Won Park, Jean Marc Tadie, Jessy Deshane, Cilina Ann Rodriguez, Jean Francois Pittet, Edward Abraham, Jaroslaw W. Zmijewski

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Alterations in metabolic and bioenergetic homeostasis contribute to sepsis-mediated organ injury. However, how AMP-activated protein kinase (AMPK), a major sensor and regulator of energy expenditure and production, affects development of organ injury and loss of innate capacity during polymicrobial sepsis remains unclear. In the present experiments, we found that cross-talk between the AMPK and GSK3β signaling pathways controls chemotaxis and the ability of neutrophils and macrophages to kill bacteria ex vivo. In mice with polymicrobial abdominal sepsis or more severe sepsis induced by the combination of hemorrhage and intraabdominal infection, administration of the AMPK activator metformin or the GSK3β inhibitor SB216763 reduced the severity of acute lung injury (ALI). Improved survival in metformin-treated septic mice was correlated with preservation of mitochondrial complex V (ATP synthase) function and increased amounts of ETC complex III and IV. Although immunosuppression is a consequence of sepsis, metformin effectively increased innate immune capacity to eradicate P. aeruginosa in the lungs of septic mice. We also found that AMPK activation diminished accumulation of the immunosuppressive transcriptional factor HIF-1α as well as the development of endotoxin tolerance in LPS-treated macrophages. Furthermore, AMPK-dependent preservation of mitochondrial membrane potential also prevented LPS-mediated dysfunction of neutrophil chemotaxis. These results indicate that AMPK activation reduces the severity of polymicrobial sepsis-induced lung injury and prevents the development of sepsis-associated immunosuppression.

Original languageEnglish
Pages (from-to)937-950
Number of pages14
JournalMolecular Medicine
Volume21
DOIs
Publication statusPublished - 2015 Nov 30
Externally publishedYes

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Glycogen Synthase Kinase 3
AMP-Activated Protein Kinases
Lung Injury
Sepsis
Metformin
Chemotaxis
Immunosuppression
Energy Metabolism
Neutrophils
Macrophages
Intraabdominal Infections
Aptitude
Acute Lung Injury
Mitochondrial Membrane Potential
Electron Transport Complex III
Wounds and Injuries
Immunosuppressive Agents
Endotoxins
Homeostasis
Adenosine Triphosphate

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

AMP-activated protein kinase and glycogen synthase kinase 3β modulate the severity of sepsis-induced lung injury. / Liu, Zhongyu; Bone, Nathaniel; Jiang, Shaoning; Park, Dae Won; Tadie, Jean Marc; Deshane, Jessy; Rodriguez, Cilina Ann; Pittet, Jean Francois; Abraham, Edward; Zmijewski, Jaroslaw W.

In: Molecular Medicine, Vol. 21, 30.11.2015, p. 937-950.

Research output: Contribution to journalArticle

Liu, Z, Bone, N, Jiang, S, Park, DW, Tadie, JM, Deshane, J, Rodriguez, CA, Pittet, JF, Abraham, E & Zmijewski, JW 2015, 'AMP-activated protein kinase and glycogen synthase kinase 3β modulate the severity of sepsis-induced lung injury', Molecular Medicine, vol. 21, pp. 937-950. https://doi.org/10.2119/molmed.2015.00198
Liu, Zhongyu ; Bone, Nathaniel ; Jiang, Shaoning ; Park, Dae Won ; Tadie, Jean Marc ; Deshane, Jessy ; Rodriguez, Cilina Ann ; Pittet, Jean Francois ; Abraham, Edward ; Zmijewski, Jaroslaw W. / AMP-activated protein kinase and glycogen synthase kinase 3β modulate the severity of sepsis-induced lung injury. In: Molecular Medicine. 2015 ; Vol. 21. pp. 937-950.
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