Amphiphilic small peptides for delivery of plasmid DNAs and siRNAs

Eun Kyoung Bang, Hanna Cho, Sean S.H. Jeon, Na Ly Tran, Dong-Kwon Lim, Wooyoung Hur, Taebo Sim

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Although various delivery systems for nucleic acids have been reported, development of an efficient and non-toxic delivery carrier is still a key subject for gene therapy. To find new efficient delivery carriers for nucleic acids, we synthesized amphiphilic peptides composed of a guanidino group, an oleyl group, and a cysteine. We prepared both linear and branched types of peptides and found that the linear peptides were superior to the branched peptides as nucleic acid carriers. Our study also suggested that the intermolecular cysteine disulfides might allow the linear peptides to form the optimal particle sizes with nucleic acids for cellular uptake. The incorporation of a benzoyl group to the linear peptide gave rise to smaller, less suitable particle size with plasmid DNA, which greatly reduced the efficiency of plasmid DNA delivery. On the other hand, the benzoyl modification maintained the optimal particle size with siRNA, and interestingly it significantly enhanced the siRNA delivery. The higher efficiency is because the hydrophobicity from the benzoyl group might assist in interacting with the hydrophobic cell membrane. This demonstrates that a small structural change can modulate the preference of the carriers. Our study may provide an insight designing efficient delivery carriers.

Original languageEnglish
JournalChemical Biology and Drug Design
DOIs
Publication statusAccepted/In press - 2017

Keywords

  • Amphiphilic peptide
  • Cationic lipid
  • DNA delivery
  • Gene therapy
  • SiRNA delivery

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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