AMPK, a metabolic sensor, is involved in isoeugenol-induced glucose uptake in muscle cells

Nami Kim, Jung Ok Lee, Hye Jeong Lee, Yong Woo Lee, Hyung Ip Kim, Su Jin Kim, Sun-Hwa Park, Chul Su Lee, Sun Woo Ryoo, Geum Sook Hwang, Hyeon Soo Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Isoeugenol exerts various beneficial effects on human health. However, the mechanisms underlying these effects are poorly understood. In this study, we observed that isoeugenol activated AMP-activated protein kinase (AMPK) and increased glucose uptake in rat L6 myotubes. Isoeugenol-induced increase in intracellular calcium concentration and glucose uptake was inhibited by STO-609, an inhibitor of calcium/calmodulin-dependent protein kinase kinase (CaMKK). Isoeugenol also increased the phosphorylation of protein kinase C-α (PKCα). Chelation of calcium with BAPTA-AM blocked isoeugenol-induced AMPK phosphorylation and glucose uptake. Isoeugenol stimulated p38MAPK phosphorylation that was inhibited after pretreatment with compound C, an AMPK inhibitor. Isoeugenol also increased glucose transporter type 4 (GLUT4) expression and its translocation to the plasma membrane. GLUT4 translocation was not observed after the inhibition of AMPK and CaMKK. In addition, isoeugenol activated the Akt substrate 160 (AS160) pathway, which is downstream of the p38MAPK pathway. Knockdown of the gene encoding AS160 inhibited isoeugenol-induced glucose uptake. Together, these results indicate that isoeugenol exerts beneficial health effects by activating the AMPK/p38MAPK/AS160 pathways in skeletal muscle.

Original languageEnglish
Pages (from-to)105-114
Number of pages10
JournalJournal of Endocrinology
Volume228
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1

Fingerprint

isoeugenol
AMP-Activated Protein Kinases
Muscle Cells
Glucose
Glucose Transporter Type 4
Phosphorylation
Calcium-Calmodulin-Dependent Protein Kinase Kinase
Gene Knockdown Techniques
Calcium
Calcium-Calmodulin-Dependent Protein Kinases

Keywords

  • AMPK
  • Glucose uptake
  • Isoeugenol
  • p38 MAPK

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

AMPK, a metabolic sensor, is involved in isoeugenol-induced glucose uptake in muscle cells. / Kim, Nami; Lee, Jung Ok; Lee, Hye Jeong; Lee, Yong Woo; Kim, Hyung Ip; Kim, Su Jin; Park, Sun-Hwa; Lee, Chul Su; Ryoo, Sun Woo; Hwang, Geum Sook; Kim, Hyeon Soo.

In: Journal of Endocrinology, Vol. 228, No. 2, 01.02.2016, p. 105-114.

Research output: Contribution to journalArticle

Kim, N, Lee, JO, Lee, HJ, Lee, YW, Kim, HI, Kim, SJ, Park, S-H, Lee, CS, Ryoo, SW, Hwang, GS & Kim, HS 2016, 'AMPK, a metabolic sensor, is involved in isoeugenol-induced glucose uptake in muscle cells', Journal of Endocrinology, vol. 228, no. 2, pp. 105-114. https://doi.org/10.1530/JOE-15-0302
Kim, Nami ; Lee, Jung Ok ; Lee, Hye Jeong ; Lee, Yong Woo ; Kim, Hyung Ip ; Kim, Su Jin ; Park, Sun-Hwa ; Lee, Chul Su ; Ryoo, Sun Woo ; Hwang, Geum Sook ; Kim, Hyeon Soo. / AMPK, a metabolic sensor, is involved in isoeugenol-induced glucose uptake in muscle cells. In: Journal of Endocrinology. 2016 ; Vol. 228, No. 2. pp. 105-114.
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