AMPK, a metabolic sensor, is involved in isoeugenol-induced glucose uptake in muscle cells

Nami Kim, Jung Ok Lee, Hye Jeong Lee, Yong Woo Lee, Hyung Ip Kim, Su Jin Kim, Sun Hwa Park, Chul Su Lee, Sun Woo Ryoo, Geum Sook Hwang, Hyeon Soo Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Isoeugenol exerts various beneficial effects on human health. However, the mechanisms underlying these effects are poorly understood. In this study, we observed that isoeugenol activated AMP-activated protein kinase (AMPK) and increased glucose uptake in rat L6 myotubes. Isoeugenol-induced increase in intracellular calcium concentration and glucose uptake was inhibited by STO-609, an inhibitor of calcium/calmodulin-dependent protein kinase kinase (CaMKK). Isoeugenol also increased the phosphorylation of protein kinase C-α (PKCα). Chelation of calcium with BAPTA-AM blocked isoeugenol-induced AMPK phosphorylation and glucose uptake. Isoeugenol stimulated p38MAPK phosphorylation that was inhibited after pretreatment with compound C, an AMPK inhibitor. Isoeugenol also increased glucose transporter type 4 (GLUT4) expression and its translocation to the plasma membrane. GLUT4 translocation was not observed after the inhibition of AMPK and CaMKK. In addition, isoeugenol activated the Akt substrate 160 (AS160) pathway, which is downstream of the p38MAPK pathway. Knockdown of the gene encoding AS160 inhibited isoeugenol-induced glucose uptake. Together, these results indicate that isoeugenol exerts beneficial health effects by activating the AMPK/p38MAPK/AS160 pathways in skeletal muscle.

Original languageEnglish
Pages (from-to)105-114
Number of pages10
JournalJournal of Endocrinology
Volume228
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1

Keywords

  • AMPK
  • Glucose uptake
  • Isoeugenol
  • p38 MAPK

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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    Kim, N., Lee, J. O., Lee, H. J., Lee, Y. W., Kim, H. I., Kim, S. J., Park, S. H., Lee, C. S., Ryoo, S. W., Hwang, G. S., & Kim, H. S. (2016). AMPK, a metabolic sensor, is involved in isoeugenol-induced glucose uptake in muscle cells. Journal of Endocrinology, 228(2), 105-114. https://doi.org/10.1530/JOE-15-0302