Amplification of resonant rayleigh light scattering response using immunogold colloids for detection of lysozyme

Phuoc Long Truong, Seung Phill Choi, Sang Jun Sim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A strategy for attomolar-level detection of small molecule-size proteins is reported based on Rayleigh light scattering spectroscopy of individual nanoplasmonic aptasensors by exploiting the outstanding characteristics of gold colloids to amplify the nontransparent resonant signal at ultralow analyte concentrations. The fabrication method utilizes thiol-mediated adsorption of a DNA aptamer on the immobilized Au nanoparticle surface, the interfacial binding characteristics of the aptamer with its target molecules, and the antibody-antigen interaction through plasmonic resonance coupling of the Au nanoparticles. Using lysozyme as a model analyte for disease detection, the detection limit of the aptasensor is ∼7 × 103 aM, corresponding to the LSPR λmax shift of ∼2.25 nm. Up to a 380% increase in the localized resonant λmax shift is demonstrated upon antibody binding to the analyte compared to the primary response during signal amplification using immunogold colloids. This enhancement leads to a limit of detection of ∼7 aM, which is an improvement of three orders of magnitude. The results demonstrate substantial promise for developing coupled plasmonic nanostructures for ultrasensitive detection of various biological and chemical analytes. An individual aptasensor is effectively exploited to detect disease utilizing thiol-mediated adsorption of the DNA aptamer, the interfacial binding characteristics of the aptamer with its target, and the antibody-antigen interaction through plasmon coupling of the nanoparticles. Up to a 380% increase in the LSPR λmax shift is demonstrated compared to an unenhanced shift using immunogold as the resonant label. This leads to a detection limit of ∼7 aM.

Original languageEnglish
Pages (from-to)3485-3492
Number of pages8
JournalSmall
Volume9
Issue number20
DOIs
Publication statusPublished - 2013 Oct 25

Fingerprint

Rayleigh scattering
Colloids
Muramidase
Antibodies
Light scattering
Nucleotide Aptamers
Nanoparticles
Amplification
Limit of Detection
Enzymes
Antigens
Light
Sulfhydryl Compounds
Adsorption
DNA
Gold Colloid
Molecules
Nanostructures
Labels
Spectrum Analysis

Keywords

  • aptasensors
  • immunogold colloids
  • LSPR
  • plasmon coupling
  • rayleigh scattering

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

Cite this

Amplification of resonant rayleigh light scattering response using immunogold colloids for detection of lysozyme. / Truong, Phuoc Long; Choi, Seung Phill; Sim, Sang Jun.

In: Small, Vol. 9, No. 20, 25.10.2013, p. 3485-3492.

Research output: Contribution to journalArticle

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