Abstract
Amplified neuronal signals ean be recorded by microelectrode array (MEA) in microfluidic channels where neurons were cultured in 3D. MEA was located at the bottom of channels where only axons, isolated from somas by collagen blocks, were grown. The impedance of microchannels is higher than that of open chambers, creating enhanced signals at electrodes compared to simple chambers. The first signals were recorded at 3 weeks during the 5 weeks of culture period. The magnitude of signals was increased at week 5 and decreased afterwards. This chip is expected to provide a useful tool for long-term cultures of neurons that can observe axon growth as well as amplified action potentials.
| Original language | English |
|---|---|
| Title of host publication | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 413-415 |
| Number of pages | 3 |
| Volume | 1 |
| ISBN (Print) | 9781632666246 |
| Publication status | Published - 2013 |
| Event | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany Duration: 2013 Oct 27 → 2013 Oct 31 |
Other
| Other | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
|---|---|
| Country | Germany |
| City | Freiburg |
| Period | 13/10/27 → 13/10/31 |
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Keywords
- 3D culture
- Long-term neuron culture
- MEA
- Microchannels
- Neuronal signals
ASJC Scopus subject areas
- Bioengineering
Cite this
Son, M., Choi, I., Chung, S., & Kang, J. Y. (2013). Amplified microelectrode recordings of neuron clusters in a three dimensional cell culture chip. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 1, pp. 413-415). Chemical and Biological Microsystems Society.