TY - GEN
T1 - Amplifying translocation signals by aqueous two-phase system in a nanopore
AU - Lee, Sang Jun
AU - Kang, Ji Yoon
AU - Choi, Wonjoon
AU - Kwak, Rhokyun
N1 - Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - Here, we demonstrate the amplification of translocation signals in a commercial nanofluidic resistive pulse sensing (RPS) system by utilizing aqueous two-phase system (ATPS). Two immiscible liquids build a liquid-liquid interface in a nanopore, so nanoparticles pass across the interface, during nanopore translocation. As transferring from high-affinity liquid into low-affinity one, the particles are coated by high-affinity liquid, inducing the increase of effective particle size. Consequently, this conformal coating can amplify the blockage current of nanoparticles. We experimentally capture that the blockage signal of 100nm polystyrene bead can be amplified 4.23-fold in ATPS (coating thickness: ∼40nm) than in a single phase solution.
AB - Here, we demonstrate the amplification of translocation signals in a commercial nanofluidic resistive pulse sensing (RPS) system by utilizing aqueous two-phase system (ATPS). Two immiscible liquids build a liquid-liquid interface in a nanopore, so nanoparticles pass across the interface, during nanopore translocation. As transferring from high-affinity liquid into low-affinity one, the particles are coated by high-affinity liquid, inducing the increase of effective particle size. Consequently, this conformal coating can amplify the blockage current of nanoparticles. We experimentally capture that the blockage signal of 100nm polystyrene bead can be amplified 4.23-fold in ATPS (coating thickness: ∼40nm) than in a single phase solution.
KW - Aqueous two-phase system(ATPS)
KW - Nanopre
KW - Resistive pulse sensing(RPS)
UR - http://www.scopus.com/inward/record.url?scp=84983347710&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84983347710
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1109
EP - 1111
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -