Abstract
Here, we demonstrate the amplification of translocation signals in a commercial nanofluidic resistive pulse sensing (RPS) system by utilizing aqueous two-phase system (ATPS). Two immiscible liquids build a liquid-liquid interface in a nanopore, so nanoparticles pass across the interface, during nanopore translocation. As transferring from high-affinity liquid into low-affinity one, the particles are coated by high-affinity liquid, inducing the increase of effective particle size. Consequently, this conformal coating can amplify the blockage current of nanoparticles. We experimentally capture that the blockage signal of 100nm polystyrene bead can be amplified 4.23-fold in ATPS (coating thickness: ∼40nm) than in a single phase solution.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1109-1111 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| Publication status | Published - 2015 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 2015 Oct 25 → 2015 Oct 29 |
Other
| Other | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country | Korea, Republic of |
| City | Gyeongju |
| Period | 15/10/25 → 15/10/29 |
Keywords
- Aqueous two-phase system(ATPS)
- Nanopre
- Resistive pulse sensing(RPS)
ASJC Scopus subject areas
- Control and Systems Engineering
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Cite this
Lee, S. J., Kang, J. Y., Choi, W., & Kwak, R. (2015). Amplifying translocation signals by aqueous two-phase system in a nanopore. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1109-1111). Chemical and Biological Microsystems Society.