Amplifying translocation signals by aqueous two-phase system in a nanopore

Sang Jun Lee, Ji Yoon Kang, Wonjoon Choi, Rhokyun Kwak

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here, we demonstrate the amplification of translocation signals in a commercial nanofluidic resistive pulse sensing (RPS) system by utilizing aqueous two-phase system (ATPS). Two immiscible liquids build a liquid-liquid interface in a nanopore, so nanoparticles pass across the interface, during nanopore translocation. As transferring from high-affinity liquid into low-affinity one, the particles are coated by high-affinity liquid, inducing the increase of effective particle size. Consequently, this conformal coating can amplify the blockage current of nanoparticles. We experimentally capture that the blockage signal of 100nm polystyrene bead can be amplified 4.23-fold in ATPS (coating thickness: ∼40nm) than in a single phase solution.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1109-1111
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

Keywords

  • Aqueous two-phase system(ATPS)
  • Nanopre
  • Resistive pulse sensing(RPS)

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Lee, S. J., Kang, J. Y., Choi, W., & Kwak, R. (2015). Amplifying translocation signals by aqueous two-phase system in a nanopore. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1109-1111). Chemical and Biological Microsystems Society.