Amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells

Yuyoung Joo, Sungji Ha, Bo Hyun Hong, Jeong A. Kim, Keun A. Chang, Hyunjeong Liew, Seonghan Kim, Woong Sun, Joung Hun Kim, Young Hae Chong, Yoo Hun Suh, Hye Sun Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Amyloid precursor protein binding protein-1 (APP-BP1) binds to the carboxyl terminus of the amyloid precursor protein (APP) and serves as the bipartite activation enzyme for the ubiquitin-like protein, NEDD8. In the present study, we explored the physiological role of APP-BP1 in the cell cycle progression of fetal neural stem cells. Our results show that cell cycle progression of the cells is arrested at the G1 phase by depletion of APP-BP1, which results in a marked decrease in the proliferation of the cells. This action of APP-BP1 is antagonistically regulated by the interaction with APP. Consistent with the evidence that APP-BP1 function is critical for cell cycle progression, the amount of APP-BP1 varies depending upon cell cycle phase, with culminating expression at S-phase. Furthermore, our FRET experiment revealed that phosphorylation of APP at threonine 668, known to occur during the G2/M phase, is required for the interaction between APP and APP-BP1. We also found a moderate ubiquitous level of APP-BP1 mRNA in developing embryonic and early postnatal brains; however, APP-BP1 expression is reduced by P12, and only low levels of APP-BP1 were found in the adult brain. In the cerebral cortex of E16 rats, substantial expression of both APP-BP1 and APP mRNAs was observed in the ventricular zone. Collectively, these results indicate that APP-BP1 plays an important role in the cell cycle progression of fetal neural stem cells, through the interaction with APP, which is fostered by phopshorylation of threonine 668.

Original languageEnglish
Article numbere14203
JournalPLoS One
Volume5
Issue number12
DOIs
Publication statusPublished - 2010 Dec 16

Fingerprint

Fetal Stem Cells
Neural Stem Cells
protein binding
amyloid
Stem cells
Amyloid
Protein Binding
stem cells
binding proteins
cell cycle
Cell Cycle
Carrier Proteins
Cells
Amyloid beta-Protein Precursor
Threonine
proteins
Brain
Ubiquitins
threonine
interphase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Joo, Y., Ha, S., Hong, B. H., Kim, J. A., Chang, K. A., Liew, H., ... Kim, H. S. (2010). Amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells. PLoS One, 5(12), [e14203]. https://doi.org/10.1371/journal.pone.0014203

Amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells. / Joo, Yuyoung; Ha, Sungji; Hong, Bo Hyun; Kim, Jeong A.; Chang, Keun A.; Liew, Hyunjeong; Kim, Seonghan; Sun, Woong; Kim, Joung Hun; Chong, Young Hae; Suh, Yoo Hun; Kim, Hye Sun.

In: PLoS One, Vol. 5, No. 12, e14203, 16.12.2010.

Research output: Contribution to journalArticle

Joo, Y, Ha, S, Hong, BH, Kim, JA, Chang, KA, Liew, H, Kim, S, Sun, W, Kim, JH, Chong, YH, Suh, YH & Kim, HS 2010, 'Amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells', PLoS One, vol. 5, no. 12, e14203. https://doi.org/10.1371/journal.pone.0014203
Joo, Yuyoung ; Ha, Sungji ; Hong, Bo Hyun ; Kim, Jeong A. ; Chang, Keun A. ; Liew, Hyunjeong ; Kim, Seonghan ; Sun, Woong ; Kim, Joung Hun ; Chong, Young Hae ; Suh, Yoo Hun ; Kim, Hye Sun. / Amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells. In: PLoS One. 2010 ; Vol. 5, No. 12.
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