An AIE-Based Probe for Rapid and Ultrasensitive Imaging of Plasma Membranes in Biosystems

Lei Shi, Yan Hong Liu, Kun Li, Amit Sharma, Kang Kang Yu, Myung Sun Ji, Ling Ling Li, Qian Zhou, Hong Zhang, Jong Seung Kim, Xiao Qi Yu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The abnormality of the plasma membrane (PM) is an important biomarker for cell status and many diseases. Hence, visualizing the PM, especially in complex systems, is an emerging field in the life sciences, especially in low-resource settings. Herein, we developed a water-soluble PM-specific probe utilizing electrostatic and hydrophobic interaction strategies with aggregation-induced emission as the signal output. The probe could image the PM with many advanced features (wash-free, ultrafast staining process, excellent PM specificity, and good biocompatibility), which were demonstrated by the PM imaging of neurons. The probe allowed for the first time the imaging of erythrocytes in the complex brain environment through a fluorescence-based method. Moreover, the PM of the epidermal and partial view of the eyeball structure of live zebrafish are also revealed.

Original languageEnglish
JournalAngewandte Chemie - International Edition
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Keywords

  • aggregation-induced emission
  • fluorescent probes
  • neurons
  • plasma membranes
  • structural biology

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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  • Cite this

    Shi, L., Liu, Y. H., Li, K., Sharma, A., Yu, K. K., Ji, M. S., Li, L. L., Zhou, Q., Zhang, H., Kim, J. S., & Yu, X. Q. (Accepted/In press). An AIE-Based Probe for Rapid and Ultrasensitive Imaging of Plasma Membranes in Biosystems. Angewandte Chemie - International Edition. https://doi.org/10.1002/anie.201909498