An alternate method for estimation of cell growth kinetics from batch cultures

Jeewon Lee, H. L. Chang, S. J. Parulekar, J. Hong

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An alternative to estimation of cell growth kinetics via continuous culture experiments is proposed in this article. The method employed is based on batch culture experiments with very small inocula (initial cell concentrations being typically less than 5000 cells/mL). Such low initial cell concentrations result in extended exponential cell growth phase during which culture conditions remain unchanged, thereby permitting precise estimation of specific cell growth rates from batch experiments especially for fast-growing microorganisms such as Bacillus species. The effectivenesss and utility of this approach are demonstrated via several experiments conducted with a wild-type strain (Bacillus subtilis TN106) and a recombinant stain (B. subtilis TN106[pAT5]). True establishment of exponential growth phase requires insignificant variance of most of the culture conditions during the initial growth phase. Satisfaction of this requirement is demonstrated for microbial systems investigated here. This approach is especially well suited for recombinant microorganisms containing segregationally unstable plasmids, since estimation of growth kinetics of these from continuous cultures is very difficult and highly unreliable due to continual reversion of recombinant cells to plasmid-free host cells unless some selection pressure is applied at levels sufficient to keep the presence of plasmid-free cells minimal.

Original languageEnglish
Pages (from-to)26-34
Number of pages9
JournalBiotechnology and Bioengineering
Volume37
Issue number1
DOIs
Publication statusPublished - 1991 Jan 5
Externally publishedYes

Fingerprint

Batch Cell Culture Techniques
Growth kinetics
Cell growth
Cell culture
Plasmids
Bacilli
Growth
Microorganisms
Experiments
Bacillus subtilis
Coloring Agents
Bacillus
Pressure

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

An alternate method for estimation of cell growth kinetics from batch cultures. / Lee, Jeewon; Chang, H. L.; Parulekar, S. J.; Hong, J.

In: Biotechnology and Bioengineering, Vol. 37, No. 1, 05.01.1991, p. 26-34.

Research output: Contribution to journalArticle

Lee, Jeewon ; Chang, H. L. ; Parulekar, S. J. ; Hong, J. / An alternate method for estimation of cell growth kinetics from batch cultures. In: Biotechnology and Bioengineering. 1991 ; Vol. 37, No. 1. pp. 26-34.
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