An ApoB100-mimetic vaccine prevents obesity and liver steatosis in ApoE-/- mice

Su Kang Kong, Moon Kyung Choe, Hyung Ji Kim, Young Sik Kim, Bert Binas, Hyo Joon Kim

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Background Recently, a peptide vaccine (B4T) was developed that prevents high fat diet (HFD)-induced obesity and liver steatosis in wild type mice and appears to target an epitope present in ApoB100 but not ApoB48. Here, we ask whether B4T remains effective in ApoE knockout (ApoE-ko) mice, which exhibit a greatly increased ApoB48/ApoB100 ratio and develop atherosclerosis under HFD. Methods HFD-fed male ApoE-ko mice were injected with B4T or vehicle 3 times between 5 and 15 weeks of age. Until 45 weeks of age, they were regularly weighed and antibody titers determined. In the end, adiposity and organ histologies were examined. Results We find that in the ApoE-ko mice, B4T prevents HFD-induced body weight increases (p < 0.01) to a comparable degree as previously shown in wild type mice. Also, liver steatosis was prevented as previously shown in wild type mice. By contrast, atherosclerotic plaque formation was not prevented in any of the vaccinated mice studied, in line with the observation that antibody production paralleled the weight reduction but largely preceded atherogenesis. Conclusion The findings demonstrate effectiveness of B4T despite the increased ApoB48/B100 ratio, but argue against an effect on de novo plaque formation. At least under the current vaccination schedule, the obesity- and atherosclerosis-related roles of ApoB appear to be dissociable.

Original languageEnglish
Pages (from-to)1140-1144
Number of pages5
JournalPharmacological Reports
Volume69
Issue number6
DOIs
Publication statusPublished - 2017 Dec 1

Keywords

  • Apolipoprotein B
  • Apolipoprotein E
  • Atherosclerosis
  • Obesity
  • Vaccine

ASJC Scopus subject areas

  • Pharmacology

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