An application of β-glycosidase to transformation of ginsenosides for the effective production of specific ginsenosides with biological efficacy

Youl Her, Young Chul Lee, Jin Hwan Oh, Yoon-E Choi, Chang Woo Lee, Jin Suk Kim, Hwan Mook Kim, Ji Won Yang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Over the past several decades, the pharmacological effects of ginsenosides in Panax ginseng roots have been extensively investigated. Here, we developed a method for producing specific ginsenosides (F1 and F2) with good yields (F1:162 mg/g, F2:305 mg/g) using β-glycosidase purified from Aspergillus niger. In addition, each ginsenoside (at least 25 species) was separated and purified by high performance liquid chromatography (HPLC) using five different types of solvents and different purification steps. In addition, the Rg3:Rh2 mixture (1:1, w/w) was shown to inhibit a specific lung cancer cell line (NCI-H232) in vivo, displaying an anticancer effect at a dose lower than achieved using treatments with single Rg3 or Rh2. This finding suggests that the combination of ginsenosides for targeting anticancer is more effective than the use of a single ginsenoside from ginseng or red ginseng.

Original languageEnglish
Pages (from-to)538-546
Number of pages9
JournalBiotechnology and Bioprocess Engineering
Volume17
Issue number3
DOIs
Publication statusPublished - 2012 Jun 1
Externally publishedYes

Fingerprint

Ginsenosides
Glycoside Hydrolases
Aspergillus
High performance liquid chromatography
Panax
Purification
Cells
Aspergillus niger
Lung Neoplasms
High Pressure Liquid Chromatography
Pharmacology
Cell Line

Keywords

  • Enzymatic process
  • Ginsenoside combination
  • Ginsenosides
  • Lung cancer cell line (NCI-H23)
  • Purification
  • Solvent system

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

Cite this

An application of β-glycosidase to transformation of ginsenosides for the effective production of specific ginsenosides with biological efficacy. / Her, Youl; Lee, Young Chul; Oh, Jin Hwan; Choi, Yoon-E; Lee, Chang Woo; Kim, Jin Suk; Kim, Hwan Mook; Yang, Ji Won.

In: Biotechnology and Bioprocess Engineering, Vol. 17, No. 3, 01.06.2012, p. 538-546.

Research output: Contribution to journalArticle

Her, Youl ; Lee, Young Chul ; Oh, Jin Hwan ; Choi, Yoon-E ; Lee, Chang Woo ; Kim, Jin Suk ; Kim, Hwan Mook ; Yang, Ji Won. / An application of β-glycosidase to transformation of ginsenosides for the effective production of specific ginsenosides with biological efficacy. In: Biotechnology and Bioprocess Engineering. 2012 ; Vol. 17, No. 3. pp. 538-546.
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