An Arabidopsis homologue of human seven-in-absentia-interacting protein is involved in pathogen resistance

Youn Sung Kim, Byung Kook Ham, Kyung Hee Paek, Chung M. Park, Nam H. Chua

Research output: Contribution to journalArticle

10 Citations (Scopus)


Human seven-in-absentia (SIAH)-interacting protein (SIP) is a component of the E3 ligase complex targeting beta-catenin for destruction. Arabidopsis has one SIP protein (AtSIP) with 32% amino acid sequence identity to SIP. To investigate the functions of AtSIP, we isolated an atsip knockout mutant, and generated transgenic plants overexpressing AtSIP. The growth rates and morphologies of the atsip and transgenic plants were indistinguishable from those of wild type. However, atsip plants were more susceptible to Pseudomonas syringae infection, and the transgenic plants overexpressing AtSIP were more resistant. Consistent with this, RNA blot analysis showed that the AtSIP gene is strongly induced by wounding and hydrogen peroxide treatment. In addition, when plants were infected with P. syringae, AtSIP was transiently induced prior to PR-1 induction. These observations show that Arabidopsis AtSIP plays a role in resistance to pathogenic infection.

Original languageEnglish
Pages (from-to)389-394
Number of pages6
JournalMolecules and Cells
Issue number3
Publication statusPublished - 2006 Jun 30



  • Arabidopsis thaliana
  • Biotic stress
  • Disease resistance
  • Hydrogen peroxide
  • SIAH interacting protein
  • Wounding

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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