An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance

Mei Hua Cui, Kyoung Shin Yoo, Sujin Hyoung, Ha Thi Kim Nguyen, Yun Young Kim, Hae Jin Kim, Sung Han Ok, Sang-Dong Yoo, Jeong Sheop Shin

Research output: Contribution to journalArticle

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Abstract

We have characterized the function of a plant R2R3-MYB transcription factor, Arabidopsis thaliana MYB20 (AtMYB20). Transgenic plants overexpressing AtMYB20 (AtMYB20-OX) enhanced salt stress tolerance while repression lines (AtMYB20-SRDX) were more vulnerable to NaCl than wild-type plants. Following NaCl treatment, the expressions of ABI1, ABI2 and AtPP2CA, which encode type 2C serine/threonine protein phosphatases (PP2Cs) that act as negative regulators in abscisic acid (ABA) signaling, were suppressed in AtMYB20-OX but induced in AtMYB20-SRDX. The electrophoretic mobility shift assay results revealed that AtMYB20 binds to the promoter regions containing the MYB recognition sequence (TAACTG) and an ACGT core element of ABI1 and AtPP2CA. These findings suggest that AtMYB20 down-regulates the expression of PP2Cs, the negative regulator of ABA signaling, and enhances salt tolerance.

Original languageEnglish
Pages (from-to)1773-1778
Number of pages6
JournalFEBS Letters
Volume587
Issue number12
DOIs
Publication statusPublished - 2013 Jun 19

Fingerprint

Salt-Tolerance
Abscisic Acid
Phosphoprotein Phosphatases
Arabidopsis
Transcription Factors
Salts
Electrophoretic mobility
Genetic Promoter Regions
Assays
Genetically Modified Plants
Electrophoretic Mobility Shift Assay
Down-Regulation

Keywords

  • Abscisic acid
  • MYB family
  • Salt stress
  • Type 2C serine/threonine protein phosphatase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance. / Cui, Mei Hua; Yoo, Kyoung Shin; Hyoung, Sujin; Nguyen, Ha Thi Kim; Kim, Yun Young; Kim, Hae Jin; Ok, Sung Han; Yoo, Sang-Dong; Shin, Jeong Sheop.

In: FEBS Letters, Vol. 587, No. 12, 19.06.2013, p. 1773-1778.

Research output: Contribution to journalArticle

Cui, Mei Hua ; Yoo, Kyoung Shin ; Hyoung, Sujin ; Nguyen, Ha Thi Kim ; Kim, Yun Young ; Kim, Hae Jin ; Ok, Sung Han ; Yoo, Sang-Dong ; Shin, Jeong Sheop. / An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance. In: FEBS Letters. 2013 ; Vol. 587, No. 12. pp. 1773-1778.
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