An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis

Liaoyuan Zhang, Raushan Singh, D. Sivakumar, Zewang Guo, Jiahuan Li, Fanbing Chen, Yuanzhi He, Xiong Guan, Yun Chan Kang, Jung Kul Lee

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20 Citations (Scopus)


Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.

Original languageEnglish
Pages (from-to)230-242
Number of pages13
JournalGreen Chemistry
Issue number1
Publication statusPublished - 2018 Jan 1


ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution

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