An efficient translational termination of human erythropoietin in Escherichia coli by altering the base following the stop codon

Sang Hyeon Kang, Sang Taek Jung, Taek Jin Kang, Ryang Guk Kim, Sang Hyuk Suh, Ji Hyoung Woo, Eun Yeol Lee, Cha Yong Choi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Each of the four nucleotides (A, C, G and T) was introduced as the base following the stop codon to investigate the effect of this fourth base on translational termination efficiency during the heterologous expression of human erythropoietin (hEPO) in E. coli. The efficiency of peptide chain termination in E. coli was markedly dependent on the fourth base. The choice of the fourth base was crucial to prevent the expression of undesirable proteins due to the translational infidelity such as frameshifting and stop codon read-through, and translational termination efficiency could be improved with adenosine as the fourth base.

Original languageEnglish
Pages (from-to)761-764
Number of pages4
JournalBiotechnology Techniques
Volume13
Issue number11
DOIs
Publication statusPublished - 1999

Keywords

  • Erythropoietin
  • Escherichia coli
  • Termination efficiency
  • Translational stop codon

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

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