An electrochemically reversible DNA switch

Byoung Yeon Won, Cheulhee Jung, Ki Soo Park, Hyun Gyu Park

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have developed a novel strategy for regulating G-quadruplex formation of a DNA sequence that relies on electrochemical reduction of Pb2+ and oxidation of Pb. The DNA aptamer sequence (PW17) forms a G-quadruplex structure through interaction with Pb2+. The electrochemical reduction of Pb2+ to Pb, which accumulates on the electrode surface, brings about destruction of the G-quadruplex structure. Subsequently by applying oxidation voltage, Pb on the electrode surface goes back to Pb2+ and released Pb2+ binds again to the non-structured free PW17 sequence resulting in reformation of the G-quadruplex structure. In this manner, a PW17 DNA sequence can be reversibly switched between a very stable G-quadruplex state and a non-structured state. The results should provide insight into the development of novel mechanical DNA nanomachines that are driven by simple electrochemical processes.

Original languageEnglish
Pages (from-to)100-103
Number of pages4
JournalElectrochemistry Communications
Volume27
DOIs
Publication statusPublished - 2013 Feb
Externally publishedYes

Keywords

  • Anodic stripping voltammetry
  • Aptamer
  • DNA switch
  • G-quadruplex
  • Nanomachine

ASJC Scopus subject areas

  • Electrochemistry

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