We have developed a novel strategy for regulating G-quadruplex formation of a DNA sequence that relies on electrochemical reduction of Pb2+ and oxidation of Pb. The DNA aptamer sequence (PW17) forms a G-quadruplex structure through interaction with Pb2+. The electrochemical reduction of Pb2+ to Pb, which accumulates on the electrode surface, brings about destruction of the G-quadruplex structure. Subsequently by applying oxidation voltage, Pb on the electrode surface goes back to Pb2+ and released Pb2+ binds again to the non-structured free PW17 sequence resulting in reformation of the G-quadruplex structure. In this manner, a PW17 DNA sequence can be reversibly switched between a very stable G-quadruplex state and a non-structured state. The results should provide insight into the development of novel mechanical DNA nanomachines that are driven by simple electrochemical processes.
|Number of pages||4|
|Publication status||Published - 2013 Feb|
- Anodic stripping voltammetry
- DNA switch
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